Print Email Facebook Twitter Differential effects of pentoxifylline on the hepatic inflammatory response in porcine liver cell cultures increase in inducible nitric oxide synthase expression Title Differential effects of pentoxifylline on the hepatic inflammatory response in porcine liver cell cultures increase in inducible nitric oxide synthase expression Author Centraal Instituut voor Voedingsonderzoek TNO Hoebe, K.H.N Gonzalez-Ramon, N. Nijmeijer, S.M. Witkamp, R.F. van Leengoed, L.A.M.G van Miert, A.S.J.P.A.M Monshouwer, M. Publication year 2001 Abstract Pentoxifylline (PTX) has been shown to exert hepatoprotective effects in various liver injury models. However, little information is available about the effect of PTX on the hepatic acute phase response. In the present study, the effect of PTX on a lipopolysaccharide (LPS)-induced acute phase response in primary porcine liver cell cultures was examined. During 72 hr of incubation with or without LPS, the ability of PTX to influence the secretion of tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), acute phase proteins, and nitric oxide (NO) was assessed. PTX completely inhibited LPS-induced TNF-α production and attenuated IL-6 only after 48 hr of incubation. In contrast, PTX potentiated NO production and the expression of inducible nitric oxide synthase (iNOS) in hepatocytes after stimulation with LPS. The increased expression of iNOS and concurrent production of NO was also observed when liver cell cultures were incubated with dibutyryl cyclic adenosine monophosphate. No effect of PTX on acute phase protein secretion was observed during 72 hr of incubation. The present results show that PTX differentially affects the endotoxin-induced inflammatory response in primary porcine liver cell cultures by suppressing TNF-α and IL-6 while potentiating NO production. © 2001 Elsevier Science Inc. Chemicals/CAS: Acute-Phase Proteins; Cyclic AMP, 60-92-4; Cytokines; Nitric Oxide Synthase Type II, EC 22.214.171.124; Nitric Oxide Synthase, EC 126.96.36.199; Nitrites; Pentoxifylline, 6493-05-6; Protective Agents Subject Acute phase proteinsCyclic adenosine monophosphateCytokinesHepatocytesInducible nitric oxide synthasePentoxifyllineAcute-Phase ProteinsAnimalsCells, CulturedCyclic AMPCytokinesCytoprotectionGene ExpressionLiverNitric Oxide SynthaseNitric Oxide Synthase Type IINitritesPentoxifyllineProtective AgentsSwine To reference this document use: http://resolver.tudelft.nl/uuid:407135b3-6c31-4d44-a13e-a37bc33f4772 DOI https://doi.org/10.1016/s0006-2952(01)00544-5 TNO identifier 236058 ISSN 0006-2952 Source Biochemical Pharmacology, 61 (61), 1137-1144 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.