Print Email Facebook Twitter Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials Title Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials Author Avantaggiato, G. Havenaar, R. Visconti, A. TNO Voeding Publication year 2004 Abstract In vitro screening of 14 adsorbent materials, including some commercial products used to detoxify Fusarium-mycotoxins, were tested in the pH range of 3-8 for deoxynivalenol (DON)- and nivalenol (NIV)-binding ability. Only activated carbon showed to be effective with binding capacities of 35.1 μmol and 8.8 μmol DON and NIV/g adsorbent, respectively, calculated from the adsorption isotherms. A dynamic laboratory model simulating the gastrointestinal (GI) tract of healthy pigs (TIM system) was used to evaluate the small-intestinal absorption of DON and NIV and the efficacy of activated carbon in reducing the relevant absorption. The in vitro intestinal absorptions of DON and NIV were 51% and 21%, respectively, as referred to 170 μg DON and 230 μg NIV ingested through contaminated (spiked) wheat. Most absorption occurred in the jejunal compartment for both mycotoxins. The inclusion of activated carbon produced a significant reduction in the intestinal mycotoxin absorption. At 2% inclusion level the absorption with respect to the intake was lowered from 51% to 28% for DON and from 21% to 12% for NIV. The binding activity of activated carbon for these trichothecenes was lower than that observed for zearalenone, a mycotoxin frequently co-occurring with them in naturally contaminated cereals. © 2004 Elsevier Ltd. All rights reserved. Subject Biology HealthPhysiological SciencesActivated carbonDeoxynivalenolDONGas chromatographyGastrointestinalGCGIHigh performance liquid chromatographyHPLCMycotoxin detoxificationNIVNivalenolTIMGastrointestinal modelZEAZearalenoneactivated carbonadsorbentmycotoxinnivalenoltrichothecene derivativevomitoxinzearalenoneanimal experimentanimal tissuearticlebinding affinitycalculationcommercial phenomenacontrolled studydetoxificationdynamicsevaluationfood contaminationFusariumgastrointestinal tractin vitro studyintestine absorptionisothermjejunummodelnonhumanpHscreeningsimulationsmall intestine absorptionswinewheatAdsorptionAnimalsCharcoalFood ContaminationHydrogen-Ion ConcentrationIntestinal AbsorptionIntestine, SmallModels, BiologicalSwineTrichothecenesFusariumTriticum aestivum To reference this document use: http://resolver.tudelft.nl/uuid:4057ea83-3c76-4fd3-a765-0bbef1b16926 DOI https://doi.org/10.1016/j.fct.2004.01.004 TNO identifier 237726 ISSN 0278-6915 Source Food and Chemical Toxicology, 42 (5), 817-824 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.