Title
Matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the anaysis of bioaerosols: development of a fast detector for airborne biological pathogens
Author
TNO Defensie en Veiligheid
van Wuijckhuijse, A.L.
Stowers, M.A.
Kleefsman, W.A.
van Baar, B.L.M.
Kients, C.E.
Marijnissen, J.C.M.
Publication year
2005
Abstract
A new aerosol time-of-flight mass spectrometer (ATOFMS) was developed for the near real-time analysis of single bioaerosol particles. The system combines ATOFMS with laser-induced fluorescence selection and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The influences of laser power and sample preparation on the quality of the mass spectra have been investigated. A narrow range of laser fluence gives the optimal signals in the mass spectra. On-line application of ferulic acid provides spectra with quality comparable to other preparation methods. The system can analyze aerosolized proteinaceous material up to 20,000 Da, which is the range of the molecular masses of marker ions of bacteria. The results of a preliminary test with spores of B. subtilis var niger show a good prospect for application in the identification of single bacteria and spores. The obtained spectra are promising, although more research is necessary to increase the resolution and accuracy of the spectra.
Subject
Aersol time-of-flight mass spectrometry
Bioaerosols
Maldi mass spectroscopy
On-line analysis
Biomarkers
Carboxylic acids
Desorption
Fluorescence
Ionization
Laser applications
Mass spectrometry
Proteins
Laser power
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry
Aerosols
Ferulic acid
Aerosol
Accuracy
Aerosol time of flight mass spectrometer
Airborne particle
Bacillus subtilis
Bacterial flora
Biosensor
Matrix assisted laser desorption ionization time of flight mass spectrometry
Nonhuman
Reliability
Ferulic acid, 1135-24-6, 24276-84-4
To reference this document use:
http://resolver.tudelft.nl/uuid:3dd2bff3-fee0-4592-8d12-a80e1fbd5b9f
DOI
https://doi.org/10.1016/j.jaerosci.2004.11.003
TNO identifier
90035
Publisher
Elsevier Ltd
ISSN
0021-8502
Source
Journal of Aerosol Science, 36 (36), 677-687
Document type
article