Title
Evaluation of two dynamic in vitro models simulating fasted and fed state conditions in the upper gastrointestinal tract (TIM-1 and tiny-TIM) for investigating the bioaccessibility of pharmaceutical compounds from oral dosage forms
Author
Verwei, M.
Minekus, M.
Zeijdner, E.
Schilderink, R.
Havenaar, R.
Publication year
2016
Abstract
Pharmaceutical research needs predictive in vitro tools for API bioavailability in humans. We evaluated two dynamic in vitro gastrointestinal models: TIM-1 and tiny-TIM. Four low-soluble APIs in various formulations were investigated in the TIM systems under fasted and fed conditions. API small-intestinal bioaccessibility profiles were evaluated between the two systems and in comparison with human data. Both TIM systems showed a higher bioaccessibility of ciprofloxacin and nifedipine during 3-4 h after dosing immediate release (IR) compared to modified release (MR) formulations. Higher bioaccessibility levels from IR formulations were observed under fasted state in the first 30-90 min in tiny-TIM as compared to TIM-1, resulting in a tmax similar to clinical data. Absence (ciprofloxacin) or presence (posaconazole) of a food effect on bioaccessibility was observed in both TIM systems in line with human data. A higher bioaccessibility of fenofibrate from nano- vs micro-particle formulation was found in both TIM systems. This dataset shows the predictive quality of the TIM systems for clinical data on API small-intestinal bioaccessibility from IR and MR formulations and food effects. Tiny-TIM provides higher throughput and better prediction for IR formulations. TIM-1 provides detailed information on site-specific release of APIs, relevant for MR formulations and food effects. © 2015 Elsevier B.V. All rights reserved.
Subject
Life Triskelion BV
MHR - Metabolic Health Research ARF - Analytical Research (Food)
ELSS - Earth, Life and Social Sciences
Food and Nutrition
Health
Healthy Living
Food effect
In vitro gastrointestinal model
Oral dosage form
Pharmaceutical bioaccessibility
TIM system
To reference this document use:
http://resolver.tudelft.nl/uuid:3d493119-1e03-4c6b-b250-87ca9a0101d6
DOI
https://doi.org/10.1016/j.ijpharm.2015.11.048
TNO identifier
530919
ISSN
0378-5173
Source
International Journal of Pharmaceutics, 498 (1-2), 178-186
Document type
article