Print Email Facebook Twitter No effect of C-reactive protein on early atherosclerosis development in apolipoprotein E*3-Leiden/human C-reactive protein transgenic mice Title No effect of C-reactive protein on early atherosclerosis development in apolipoprotein E*3-Leiden/human C-reactive protein transgenic mice Author Trion, A. de Maat, M.P.M. Jukema, J.W. van der Laarse, A. Maas, M.C. Offerman, E.H. Havekes, L.M. Szalai, A.J. Princen, H.M.G. Emeis, J.J. TNO Kwaliteit van Leven Publication year 2005 Abstract Objective - C-reactive protein (CRP) has been associated with risk of cardiovascular disease. It is not clear whether CRP is causally involved in the development of atherosclerosis. Mouse CRP is not expressed at high levels under normal conditions and increases in concentration only several-fold during an acute phase response. Because the dynamic range of human CRP is much larger, apolipoprotein E*3-Leiden (E3L) transgenic mice carrying the human CRP gene offer a unique model to study the role(s) of CRP in atherosclerosis development. Methods and Results - Atherosclerosis development was studied in 15 male and 15 female E3L/CRP mice; E3L transgenic littermates were used as controls. The mice were fed a hypercholesterolemic diet to induce atherosclerosis development. Cholesterol exposure did not differ between E3L/CRP and E3L mice. Plasma CRP levels were on average 10.2±6.5 mg/L in male E3L/CRP mice, 0.2±0.1 mg/L in female E3L/CRP mice, and undetectable in E3L mice. Quantification of atherosclerosis showed that lesion area in E3L/CRP mice was not different from that in E3L mice. Conclusion - This study demonstrates that mildly elevated levels of CRP in plasma do not contribute to the development of early atherosclerosis in hypercholesterolemic E3L/CRP mice. © 2005 American Heart Association, Inc. Chemicals / CAS: C reactive protein, 9007-41-4; cholesterol, 57-88-5; Apolipoprotein E3; Apolipoproteins E; Biological Markers; C-Reactive Protein, 9007-41-4; Cholesterol, 57-88-5 Subject BiologyBiomedical ResearchAtherosclerosisC-reactive proteinInflammationMouseTransgeneAcute phase proteinApolipoprotein E3 LeidenBiological markerC reactive proteinCholesterolUnclassified drugAnimal experimentAnimal modelAnimal tissueAtherogenesisBlood samplingCardiovascular riskCholesterol blood levelCholesterol dietControlled studyEnzyme linked immunosorbent assayExperimental modelHistopathologyHypercholesterolemiaImmunohistochemistryInflammationMouse strainNonhumanPathophysiologyProtein blood levelProtein expressionProtein functionTransgenic mouseAnimalsApolipoprotein E3Apolipoproteins EAtherosclerosisBiological MarkersBody WeightC-Reactive ProteinCholesterolEarly DiagnosisEatingEndothelium, VascularFemaleHumansHypercholesterolemiaMaleMiceMice, TransgenicMonocytesRisk FactorsSeverity of Illness Index To reference this document use: http://resolver.tudelft.nl/uuid:3ce7dae9-32f4-4fab-8791-9a10fb1dd6dd DOI https://doi.org/10.1161/01.atv.0000171992.36710.1e TNO identifier 238640 ISSN 1079-5642 Source Arteriosclerosis, Thrombosis, and Vascular Biology, 25 (8), 1635-1640 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.