Print Email Facebook Twitter Replication-deficient human adenovirus type 35 vectors for gene transfer and vaccination: Efficient human cell infection and bypass of preexisting adenovirus immunity Title Replication-deficient human adenovirus type 35 vectors for gene transfer and vaccination: Efficient human cell infection and bypass of preexisting adenovirus immunity Author Vogels, R. Zuijdgeest, D. van Rijnsoever, R. Hartkoorn, E. Damen, I. de Béthune, M.-P. Kostense, S. Penders, G. Helmus, N. Koudstaal, W. Cecchini, M. Wetterwald, A. Sprangers, M. Lemckert, A. Ophorst, O. Koel, B. van Meerendonk, M. Quax, P. Panitti, L. Grimbergen, J. Bout, A. Goudsmit, J. Havenga, M. Publication year 2003 Abstract Replication-deficient human adenovirus type 5 (Ad5) can be produced to high titers in complementing cell lines, such as PER.C6, and is widely used as a vaccine and gene therapy vector. However, preexisting immunity against Ad5 hampers consistency of gene transfer, immunological responses, and vector-mediated toxicities. We report the identification of human Ad35 as a virus with low global prevalence and the generation of an Ad35 vector plasmid system for easy insertion of heterologous genes. In addition, we have identified the minimal sequence of the Ad35-E1B region (molecular weight, 55,000 [55K]), pivotal for complementation of fully E1-lacking Ad35 vector on PER.C6 cells. After stable insertion of the 55K sequence into PER.C6 cells a cell line was obtained (PER.C6/55K) that efficiently transcomplements both Ad5 and Ad35 vectors. We further demonstrate that transduction with Ad35 is not hampered by preexisting Ad5 immunity and that Ad35 efficiently infects dendritic cells, smooth muscle cells, and synoviocytes, in contrast to Ad5. Chemicals/CAS: Adenovirus E1B Proteins; Antibodies, Viral Subject BiologyBiomedical ResearchAdenovirus vectorAnimal cellControlled studyDendritic cellGene insertionGene sequenceGene therapyGene transferGenetic complementationGenetic transductionHumanHuman adenovirusHuman adenovirus 35Human adenovirus 5Human cellImmune responseMouseNonhumanPlasmidPrevalenceSmooth muscle fiberSynoviocyteVaccinationVirus immunityVirus replicationAdenovirus E1B ProteinsAdenoviruses, HumanAnimalsAntibodies, ViralCell LineCells, CulturedDendritic CellsGene Transfer TechniquesGenetic VectorsHumansMiceMice, Inbred BALB CMuscle, SmoothNeutralization TestsPlasmidsSynovial MembraneVaccinationVirus AssemblyVirus Replication To reference this document use: http://resolver.tudelft.nl/uuid:3cc940b7-ffea-4a83-ad14-9d7e666d4e55 DOI https://doi.org/10.1128/jvi.77.15.8263-8271.2003 TNO identifier 237213 ISSN 0022-538X Source Journal of Virology, 77 (15), 8263-8271 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.