Print Email Facebook Twitter Probing the proteome response to toluene exposure in the solvent tolerant pseudomonas putida S12 Title Probing the proteome response to toluene exposure in the solvent tolerant pseudomonas putida S12 Author Wijte, D. van Baar, B.L.M. Heck, A.J.R. Altelaar, A.F.M. Publication year 2011 Abstract To enhance target production from biocatalysts, it is necessary to thoroughly understand the molecular mechanisms involved in production, degradation, and, importantly, adaptation to the required environment. One such bacterium with high potential for biocatalysis is the solvent-tolerant bacteria Pseudomonas putida S12, which, among others, is able to degrade organic solvents. For bioconversion of organic solvents to become a successful industrial process, the understanding of the molecular response upon solvent tolerance is essential. Here we performed a quantitative analysis of the P. putida S12 proteome at different stages of adaptation to toluene. Using a stable isotope dimethylation labeling approach we monitored the differential expression of 528 proteins, including often hard-to-detect membrane associate proteins, such as multiple RND-family transporters and ABC transporters of nutrients. Our quantitative proteomics approach revealed the remarkable ability of P. putida S12 to severely change its protein expression profile upon toluene exposure. This proteome response entails a significant increase in energy metabolism and expression of the solvent efflux pump SrpABC, confirming its role in solvent tolerance. Other proteins strongly up-regulated in the presence of toluene include the multidrug efflux membrane protein PP1272 and the cation/acetate symporter ActP and may form interesting alternative targets for improving solvent tolerance. © 2011 American Chemical Society. Subject LifeCBRN - CBRN ProtectionEELS - Earth, Environmental and Life SciencesBiocatalysisEfflux pumpsOrganic solvent toleranceP. putida S12Quantitative proteomicsStable isotope dimethyl labelingTolueneABC transporterAcetate symporterCotransporterIsotopeMembrane proteinOrganic solventTolueneUnclassified drugArticleBiocatalysisBiotransformationControlled studyDegradation kineticsDemethylationEnergy metabolismMolecular probeNonhumanNutrientPriority journalProtein expressionProteomicsPseudomonas putidaQuantitative analysisUpregulationChemicals/CAS: toluene, 108-88-3 To reference this document use: http://resolver.tudelft.nl/uuid:3baa7ea5-3b85-42d0-8f6e-825eebf2f0d2 DOI https://doi.org/10.1021/pr100401n TNO identifier 429751 ISSN 1535-3893 Source Journal of Proteome Research, 10 (2), 394-403 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.