Title
Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma
Author
He, M.
van Wijk, E.
Berger, R.
Wang, M.
Strassburg, K.
Schoeman, J.C.
Vreeken, R.J.
van Wietmarschen, H.
Harms, A.C.
Kobayashi, M.
Hankemeier, T.
van der Greef, J.
Publication year
2015
Abstract
Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group. The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analyses were applied. The results in univariate Student's t-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-B because of insufficient PPAR-gamma ligands. © 2015 Min He et al.
Subject
ELSS - Earth, Life and Social Sciences
Life
Healthy Living
Biomedical Innovation
Collagen type 2
Lipopolysaccharide
Oxylipin
Animal experiment
Animal model
Arthritis
Articular cartilage
Controlled study
Electrospray mass spectrometry
High performance liquid chromatography
Male
Mouse
Nonhuman
Oxidative stress
Tandem mass spectrometry
MSB - Microbiology and Systems Biology
To reference this document use:
http://resolver.tudelft.nl/uuid:3b3bcc66-4ccc-46be-8185-23ca94457a20
DOI
https://doi.org/10.1155/2015/543541
TNO identifier
529706
ISSN
0962-9351
Source
Mediators of Inflammation, 2015 (2015)
Document type
article