Title
Molecular cloning, functional characterization and expression analysis of a novel monosaccharide transporter gene OsMST6 from rice (Oryza sativa L.)
Author
Wang, Y.
Xiao, Y.
Zhang, Y.
Chai, C.
Wei, G.
Wei, X.
Xu, H.
Wang, M.
Ouwerkerk, P.B.F.
Zhu, Z.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
Monosaccharides transporters play important roles in assimilate supply for sink tissue development. In this study, a new monosaccharide transporter gene OsMST6 was identified from rice (Oryza sativa L.). The predicted OsMST6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter OsMST3. Heterologous expression in yeast (Saccharomyces cerevisiae) demonstrated that OsMST6 is a broad-spectrum monosaccharide transporter, with a K m of 266.1 μΜ for glucose. OsMST6-green fluorescent protein fusion protein is localized to the plasma membrane in plant. Semi-quantitative RT-PCR analysis exhibited that OsMST6 is expressed in all tested organs/tissues. In developing seeds, OsMST6 expression level is high at the early and middle grain filling stages and gradually declines later. Further analysis detected its expression in both maternal and filial tissues. RNA in situ hybridization analysis indicated that OsMST6 is predominantly expressed in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm of young seeds, in mesophyll cells of source leaf blades, and in pollens and the connective vein of anthers. In addition, OsMST6 expression is up-regulated by salt stress and sugars. The physiological role of OsMST6 for seed development and its roles in other sink and source tissues are discussed. © 2008 Springer-Verlag.
Subject
Biomedical Research
cDNA cloning
Expression pattern
Monosaccharide transporter
Oryza
Seed development
complementary DNA
glucose transporter
hybrid protein
messenger RNA
plant DNA
vegetable protein
article
DNA sequence
gene expression
genetics
in situ hybridization
isolation and purification
molecular cloning
molecular genetics
nucleotide sequence
phylogeny
physiology
plant gene
reverse transcription polymerase chain reaction
rice
Saccharomyces cerevisiae
sequence alignment
Base Sequence
Cloning, Molecular
DNA, Complementary
DNA, Plant
Gene Expression
Genes, Plant
In Situ Hybridization
Molecular Sequence Data
Monosaccharide Transport Proteins
Oryza sativa
Phylogeny
Plant Proteins
Recombinant Fusion Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Saccharomyces cerevisiae
Sequence Alignment
Sequence Analysis, DNA
Oryza
Oryza sativa
Saccharomyces cerevisiae
To reference this document use:
http://resolver.tudelft.nl/uuid:3a70501c-efd9-493c-9de8-d34783c91ffd
DOI
https://doi.org/10.1007/s00425-008-0755-8
TNO identifier
240970
ISSN
0032-0935
Source
Planta, 228 (4), 525-535
Document type
article