Print Email Facebook Twitter Chronic quercetin exposure affects fatty acid catabolism in rat lung Title Chronic quercetin exposure affects fatty acid catabolism in rat lung Author de Boer, V.C.J. van Schothorst, E.M. Dihal, A.A. van der Woude, H. Arts, I.C.W. Rietjens, I.M.C.M. Hollman, P.C.H. Keijer, J. TNO Kwaliteit van Leven Publication year 2006 Abstract Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1% quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by the long-term quercetin intervention. Up-regulation of genes (Hmgcs2, Ech1, Acox1, Pcca, Lpl and Acaa2) was verified and confirmed by quantitative real time PCR. In addition, free fatty acid levels were decreased in rats fed the quercetin diet, confirming that quercetin affects fatty acid catabolism. This in vivo study demonstrates for the first time that fatty acid catabolism is a relevant process that is affected in rats by chronic dietary quercetin. © Birkhäuser Verlag, 2006. Molecular Sequence Numbers: GENBANK: AF217591, AI014074, AI236795, AW530584, AW917667, AY234182, AY331040, BC061782, BC092586, BF281337, BF284618, BF406174, CA510796, CB546044, M84148, NM_012588, NM_012598, NM_013168, NM_013224, NM_017321, NM_017340, NM_019179, NM_022594, NM_057102, NM_130433, NM_138896, NM_173094, S81289, X55180, XM_213425, XM_232194, XM_234686, XM_234745, XM_234749, XM_234878, XM_341195, XM_341383, XM_342500, XM_342742, XM_345742, XM_345750, XM_345754, XM_345756, XM_575533, XM_575534, XM_578308, XM_578339, Z75902, Z93359, Z93363; Chemicals / CAS: quercetin, 117-39-5; Fatty Acids; Quercetin, 117-39-5 Subject Biomedical ResearchFatty acid metabolismFlavonoidsMicroarrayNutrigenomicsPolyphenolsQuercetinfatty acidquercetinanimal experimentarticlecatabolismcontrolled studydietgenomicshealthketogenesislung parenchymamalemicroarray analysisnonhumannucleotide sequenceoxidationquantitative analysisratreal time polymerase chain reactionunindexed sequenceupregulationAnimalsDietFatty AcidsGene Expression RegulationLungMaleOligonucleotide Array Sequence AnalysisQuercetinRatsRats, Inbred F344 To reference this document use: http://resolver.tudelft.nl/uuid:3a5a9129-dfee-4ccf-9153-e421b9331c3c DOI https://doi.org/10.1007/s00018-006-6316-z TNO identifier 239580 ISSN 1420-682X Source Cellular and Molecular Life Sciences, 63 (23), 2847-2858 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.