Print Email Facebook Twitter Kinetic characteristics of acidic and alkaline ceramidase in human epidermis Title Kinetic characteristics of acidic and alkaline ceramidase in human epidermis Author Houben, E. Uchida, Y. Nieuwenhuizen, W.F. de Paepe, K. Vanhaecke, T. Holleran, W.M. Rogiers, V. TNO Kwaliteit van Leven Publication year 2007 Abstract It has recently become evident that at least five ceramidase (CDase) isoforms are present in human epidermis, and that specifically acidic CDase (aCDase) and alkaline CDase (alkCDase) activities increase during keratinocyte differentiation, and thus might play a pivotal role(s) in permeability barrier function. Prior to investigating their possible roles in the epidermal barrier function, it is necessary to characterize basic kinetic parameters for these enzymes, as well as to determine the effects of the established CDase inhibitors and their activities. In this study, assays for both aCDase and alkCDase activities in fully differentiated human epidermis were optimized using a radiolabeled substrate. These studies revealed that aCDase activity is substantially higher than alkCDase activity, and that both isoenzymes are inhibited by a CDase inhibitor N-oleylethanolamine. These findings were also confirmed using an in situ enzyme assay. Copyright © 2007 S. Karger AG. Subject NutritionFood technologyCeramidaseEpidermisKinetic parametersacylsphingosine deacylaseenzyme inhibitorethanolamine derivativeisoenzymeadultarticleenzyme activityenzyme analysisenzyme assayenzyme inhibitionenzyme kineticsenzyme substrateenzymologyepidermisfemalehumanhuman tissueisoenzyme analysisisotope labelingnormal humannucleotide sequencepriority journalzymographyAdultAmidohydrolasesEnzyme RepressionEpidermisFemaleHumansHydrogen-Ion ConcentrationIsoenzymesKineticsMiddle AgedSkin Absorption To reference this document use: http://resolver.tudelft.nl/uuid:3a54d3be-7e7c-4f72-b385-33d75248a5a8 DOI https://doi.org/10.1159/000101388 TNO identifier 240021 ISSN 1660-5527 Source Skin Pharmacology and Physiology, 20 (4), 187-194 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.