Title
Endogenous collagen influences differentiation of human multipotent mesenchymal stromal cells
Author
Fernandes, H.
Mentink, A.
Bank, R.
Stoop, R.
van Blitterswijk, C.
de Boer, J.
TNO Kwaliteit van Leven
Publication year
2010
Abstract
Human multipotent mesenchymal stromal cells (hMSCs) are multipotent cells that, in the presence of appropriate stimuli, can differentiate into different lineages such as the osteogenic, chondrogenic, and adipogenic lineages. In the presence of ascorbic acid, MSCs secrete an extracellular matrix mainly composed of collagen type I. Here we assessed the potential role of endogenous collagen synthesis in hMSC differentiation and stem cell maintenance. We observed a sharp reduction in proliferation rate of hMSCs in the absence of ascorbic acid, concomitant with a reduction in osteogenesis in vitro and bone formation in vivo. In line with a positive role for collagen type I in osteogenesis, gene expression profiling of hMSCs cultured in the absence of ascorbic acid demonstrated increased expression of genes involved in adipogenesis and chondrogenesis and a reduction in expression of osteogenic genes. We also observed that matrix remodeling and anti-osteoclastogenic signals were high in the presence of ascorbic acid. The presence of collagen type I during the expansion phase of hMSCs did not affect their osteogenic and adipogenic differentiation potential. In conclusion, the collagenous matrix supports both proliferation and differentiation of osteogenic hMSCs but, on the other hand, presents signals stimulating matrix remodeling and inhibiting osteoclastogenesis. © 2010 Mary Ann Liebert, Inc.
Subject
Biology
Biomedical Research
Adipogenesis
Adipogenic
Ascorbic acids
Bone formation
Chondrogenesis
Chondrogenic
Collagen synthesis
Collagen type I
Collagenous matrix
Expansion phase
Extracellular matrices
Gene expression profiling
In-line
In-vitro
In-vivo
Matrix remodeling
Mesenchymal stromal cells
Multipotent cells
Osteoclastogenesis
Osteogenesis
Osteogenic
Proliferation rate
Stem cell
Cell culture
Collagen
Ketones
Organic acids
Stem cells
Gene expression
To reference this document use:
http://resolver.tudelft.nl/uuid:3a348206-2c44-47fc-a36c-89b1f4f7bc9f
TNO identifier
364441
ISSN
1937-3341
Source
Tissue Engineering - Part A, 16 (5), 1693-1702
Document type
article