Title
The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA
Author
Boot, H.J.
Kolen, C.P.A.M.
Andreadaki, F.J.
Leer, R.J.
Pouwels, P.H.
Prins Maurits Laboratorium TNO
Publication year
1996
Abstract
S-proteins are proteins which form a regular structure (S-layer) on the outside of the cell walls of many bacteria. Two S-protein-encoding genes are located in opposite directions on a 6.0-kb segment of the chromosome of Lactobacillus acidophilus ATCC 4356 bacteria. Inversion of this chromosomal segment occurs through recombination between two regions with identical sequences, thereby interchanging the expressed and the silent genes. In this study, we show that the region involved in recombination also has a function in efficient S-protein production. Two promoter sequences are present in the S-protein gene expression site, although only the most downstream promoter (P-1) is used to direct mRNA synthesis. S-protein mRNA directed by this promoter has a half-life of 15 min. Its untranslated leader can form a stable secondary structure in which the 5' end is base paired, whereas the ribosome- binding site is exposed. Truncation of this leader sequence results in a reduction in protein production, as shown by reporter gene analysis of Lactobacillus casei. The results obtained indicate that the untranslated leader sequence of S-protein mRNA is involved in efficient S-protein production.
Subject
Biology
Bacterial virulence
Epithelium cell
Gene expression regulation
Genetic analysis
Genetic recombination
Nonhuman
Priority journal
Promoter region
Protein secondary structure
Protein stability
Ribosome subunit
Rna sequence
Rna transcription
Bacterial Outer Membrane Proteins
Bacterial Proteins
Base Sequence
Cell Wall
Consensus Sequence
Gene Expression
Gene Expression Regulation, Bacterial
Lactobacillus acidophilus
Lactobacillus casei
Membrane Glycoproteins
Membrane Proteins
Molecular Sequence Data
Nucleic Acid Conformation
Promoter Regions (Genetics)
Recombination, Genetic
RNA, Bacterial
RNA, Messenger
Species Specificity
Transcription, Genetic
Bacteria (microorganisms)
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http://resolver.tudelft.nl/uuid:3a0a4256-465c-4fe7-b8a7-0d442eee74d6
TNO identifier
233470
ISSN
0021-9193
Source
Journal of Bacteriology, 178 (18), 5388-5394
Document type
article