Title
Comparison of antibody responses to hen's egg and cow's milk proteins in orally sensitized rats and food-allergic patients
Author
Knippels, L.M.J.
van der Kleij, H.P.M.
Koppelman, S.J.
Houben, G.F.
Penninks, A.H.
Felius, A.A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
Background: No adequate enteral sensitization models are available to study food allergy and the allergenicity of food proteins. To further validate an enteral brown Norway (BN) rat sensitization model under development, we studied specific protein recognition to determine whether a comparable pattern of proteins is recognized by the rat immune system and the human immune system. Methods: The animals were exposed to either ovalbumin as a positive reference control, hen's egg-white-protein extract, or a cow's milk preparation by daily gavage dosing (0.5, 1, 2.5, 5, 10, or 15 mg protein per rat/day) for 9 weeks. No adjuvants were used during the sensitization studies. The specificities of antibodies against hen's egg-white proteins or cow's-milk proteins in sera from orally sensitized rats and food-allergic patients were studied and compared by immunoblotting. Results: The IgG and IgE antibodies to hen's egg-white proteins and cow's-milk proteins present in sera from orally sensitized rats and food-allergic patients showed a comparable pattern of protein recognition. Conclusions: Upon daily intragastric exposure to food allergens, the specificities of the induced antibody responses in the BN rat resemble those found in food-allergic patients. These studies add further support to the hypothesis that the BN rat may provide a suitable animal model for food allergy research and research on the allergenicity of food proteins.Chemicals/CAS: Allergens; Dietary Proteins; Immunoglobulin E, 37341-29-0; Milk Proteins; Ovalbumin, 9006-59-1
Subject
Nutrition
Allergic patients
Brown Norway
Cow's milk
Food allergy
Hen's egg white
IgE
Rat
Sensitization
Egg protein
Egg white
Food allergen
Immunoglobulin E antibody
Immunoglobulin G antibody
Milk protein
Ovalbumin
Allergenicity
Animal experiment
Animal model
Antibody response
Antibody specificity
Antigen recognition
Child
Clinical article
Controlled study
Food allergy
Human
Immunoblotting
Infant
Male
Milk allergy
Nonhuman
Priority journal
Protein intake
Rat
Sensitization
Allergens
Animals
Antibody Specificity
Blotting, Western
Child
Child, Preschool
Dietary Proteins
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Food Hypersensitivity
Humans
Immune System
Immunoglobulin E
Infant
Male
Milk
Milk Hypersensitivity
Milk Proteins
Ovalbumin
Passive Cutaneous Anaphylaxis
Rats
Rats, Inbred BN
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TNO identifier
88905
ISSN
0105-4538
Source
Allergy: European Journal of Allergy and Clinical Immunology, 55 (3), 251-258
Document type
article