Print Email Facebook Twitter Comparison of antibody responses to hen's egg and cow's milk proteins in orally sensitized rats and food-allergic patients Title Comparison of antibody responses to hen's egg and cow's milk proteins in orally sensitized rats and food-allergic patients Author Knippels, L.M.J. van der Kleij, H.P.M. Koppelman, S.J. Houben, G.F. Penninks, A.H. Felius, A.A. Centraal Instituut voor Voedingsonderzoek TNO Publication year 2000 Abstract Background: No adequate enteral sensitization models are available to study food allergy and the allergenicity of food proteins. To further validate an enteral brown Norway (BN) rat sensitization model under development, we studied specific protein recognition to determine whether a comparable pattern of proteins is recognized by the rat immune system and the human immune system. Methods: The animals were exposed to either ovalbumin as a positive reference control, hen's egg-white-protein extract, or a cow's milk preparation by daily gavage dosing (0.5, 1, 2.5, 5, 10, or 15 mg protein per rat/day) for 9 weeks. No adjuvants were used during the sensitization studies. The specificities of antibodies against hen's egg-white proteins or cow's-milk proteins in sera from orally sensitized rats and food-allergic patients were studied and compared by immunoblotting. Results: The IgG and IgE antibodies to hen's egg-white proteins and cow's-milk proteins present in sera from orally sensitized rats and food-allergic patients showed a comparable pattern of protein recognition. Conclusions: Upon daily intragastric exposure to food allergens, the specificities of the induced antibody responses in the BN rat resemble those found in food-allergic patients. These studies add further support to the hypothesis that the BN rat may provide a suitable animal model for food allergy research and research on the allergenicity of food proteins.Chemicals/CAS: Allergens; Dietary Proteins; Immunoglobulin E, 37341-29-0; Milk Proteins; Ovalbumin, 9006-59-1 Subject NutritionAllergic patientsBrown NorwayCow's milkFood allergyHen's egg whiteIgERatSensitizationEgg proteinEgg whiteFood allergenImmunoglobulin E antibodyImmunoglobulin G antibodyMilk proteinOvalbuminAllergenicityAnimal experimentAnimal modelAntibody responseAntibody specificityAntigen recognitionChildClinical articleControlled studyFood allergyHumanImmunoblottingInfantMaleMilk allergyNonhumanPriority journalProtein intakeRatSensitizationAllergensAnimalsAntibody SpecificityBlotting, WesternChildChild, PreschoolDietary ProteinsElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayFood HypersensitivityHumansImmune SystemImmunoglobulin EInfantMaleMilkMilk HypersensitivityMilk ProteinsOvalbuminPassive Cutaneous AnaphylaxisRatsRats, Inbred BN To reference this document use: http://resolver.tudelft.nl/uuid:39086627-eaf1-4139-83e1-59b5eb1538bf TNO identifier 88905 ISSN 0105-4538 Source Allergy: European Journal of Allergy and Clinical Immunology, 55 (3), 251-258 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.