Title
Cultured human adult microglia from different donors display stable cytokine, chemokine and growth factor gene profiles but respond differently to a pro-inflammatory stimulus
Author
Meeuwsen, S.
Bsibsi, M.
Persoon-Deen, C.
Ravid, R.
van Noort, J.M.
TNO Preventie en Gezondheid
Publication year
2005
Abstract
Objectives: Brain microglia are highly responsive cells in the central nervous system that exert key functions in host defense as well as in neuroprotection and regeneration. In this study the gene expression profiles for 268 cytokines, chemokines, growth factors and their receptors were examined in cultures of purified human adult microglia, using cDNA array profiling. Methods: Microglia from 9 different donors were compared, also following challenge of such microglia with the pro-inflammatory cytokines TNF-α and IFN-γ. Results: A stable pattern was observed of genes abundantly expressed in the different cultures under standard conditions. Genes abundantly expressed in all microglia cultures include CCL2 (MCP-1), thymosin β-10, migration-inhibitory factor-related protein 8 (MRP8), MRP14, corticotropin-releasing factor receptor 1 and endothelin 2. Abundant gene products novel to microglia were neuromodulin (GAP43) and FltS ligand. Yet, treatment with TNF-α and IFN-γ led to widely different response profiles among the different cultures. Conclusion: These data show a surprising level of heterogeneity among human adult microglia cultures in their response to a pro-inflammatory stimulus despite the standardized methodology to examine this response. Copyright © 2005 S. Karger AG. Chemicals / CAS: Flt3 ligand, 171404-15-2; gamma interferon, 82115-62-6; migration inhibition factor, 125523-65-1; thymosin beta10, 87397-91-9; Chemokines; Cytokines; Growth Substances; Inflammation Mediators; Interferon Type II, 82115-62-6; RNA, Messenger; Tumor Necrosis Factor-alpha
Subject
Growth factor
Microglia
complementary DNA
corticotropin releasing factor receptor 1
endothelin 2
Flt3 ligand
gamma interferon
migration inhibition factor
neuromodulin
thymosin beta10
tumor necrosis factor alpha
cell culture
comparative study
controlled study
DNA microarray
gene expression
human
human cell
microglia
stimulus response
Aged
Aged, 80 and over
Cells, Cultured
Chemokines
Cytokines
Female
Gene Expression Profiling
Gene Expression Regulation
Growth Substances
Humans
Inflammation Mediators
Interferon Type II
Male
Microglia
Middle Aged
Neuroimmunomodulation
Oligonucleotide Array Sequence Analysis
RNA, Messenger
Tumor Necrosis Factor-alpha
Up-Regulation
Variation (Genetics)
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http://resolver.tudelft.nl/uuid:36da428d-e2ca-43b2-a4a3-0888f7297755
DOI
https://doi.org/10.1159/000085655
TNO identifier
238600
ISSN
1021-7401
Source
NeuroImmunoModulation, 12 (4), 235-245
Document type
article