Title
Photon counts statistics in leukocyte cell dynamics
Author
van Wijk, E.
van der Greef, J.
van Wijk, R.
Publication year
2011
Abstract
In the present experiment ultra-weak photon emission/ chemiluminescence from isolated neutrophils was recorded. It is associated with the production of reactive oxygen species (ROS) in the "respiratory burst" process which can be activated by PMA (Phorbol 12-Myristate 13-Acetate). Commonly, the reaction is demonstrated utilizing the enhancer luminol. However, with the use of highly sensitive photomultiplier equipment it is also recorded without enhancer. In that case, it can be hypothesized that photon count statistics may assist in understanding the underlying metabolic activity and cooperation of these cells. To study this hypothesis leukocytes were stimulated with PMA and increased photon signals were recorded in the quasi stable period utilizing Fano factor analysis at different window sizes. The Fano factor is defined by the variance over the mean of the number of photon within the observation time. The analysis demonstrated that the Fano factor of true signal and not of the surrogate signals obtained by random shuffling increases when the window size increased. It is concluded that photon count statistics, in particular Fano factor analysis, provides information regarding leukocyte interactions. It opens the perspective to utilize this analytical procedure in (in vivo) inflammation research. However, this needs further validation.
Subject
Life
MSB - Microbiology and Systems Biology
EELS - Earth, Environmental and Life Sciences
Sustainable Chemical Industry
Physics
Industrial Innovation
Analytical procedure
Cell dynamics
Fano factor
Highly sensitive
In-vivo
Inflammation research
Luminols
Metabolic activity
Phorbol 12-myristate 13-acetates
Photon count
Photon emissions
Reactive oxygen species
Respiratory burst
Window Size
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DOI
https://doi.org/10.1088/1742-6596/329/1/012021
TNO identifier
452792
ISSN
1742-6588
Source
Journal of Physics : Conference Series, 329 (1), 1-12
Article number
012021
Document type
article