Print Email Facebook Twitter Photon counts statistics in leukocyte cell dynamics Title Photon counts statistics in leukocyte cell dynamics Author van Wijk, E. van der Greef, J. van Wijk, R. Publication year 2011 Abstract In the present experiment ultra-weak photon emission/ chemiluminescence from isolated neutrophils was recorded. It is associated with the production of reactive oxygen species (ROS) in the "respiratory burst" process which can be activated by PMA (Phorbol 12-Myristate 13-Acetate). Commonly, the reaction is demonstrated utilizing the enhancer luminol. However, with the use of highly sensitive photomultiplier equipment it is also recorded without enhancer. In that case, it can be hypothesized that photon count statistics may assist in understanding the underlying metabolic activity and cooperation of these cells. To study this hypothesis leukocytes were stimulated with PMA and increased photon signals were recorded in the quasi stable period utilizing Fano factor analysis at different window sizes. The Fano factor is defined by the variance over the mean of the number of photon within the observation time. The analysis demonstrated that the Fano factor of true signal and not of the surrogate signals obtained by random shuffling increases when the window size increased. It is concluded that photon count statistics, in particular Fano factor analysis, provides information regarding leukocyte interactions. It opens the perspective to utilize this analytical procedure in (in vivo) inflammation research. However, this needs further validation. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesSustainable Chemical IndustryPhysicsIndustrial InnovationAnalytical procedureCell dynamicsFano factorHighly sensitiveIn-vivoInflammation researchLuminolsMetabolic activityPhorbol 12-myristate 13-acetatesPhoton countPhoton emissionsReactive oxygen speciesRespiratory burstWindow Size To reference this document use: http://resolver.tudelft.nl/uuid:35ad9d05-16c4-4620-a826-11b6fdc9950a DOI https://doi.org/10.1088/1742-6596/329/1/012021 TNO identifier 452792 ISSN 1742-6588 Source Journal of Physics : Conference Series, 329 (1), 1-12 Article number 012021 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.