Title
Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori
Author
Lokman, B.C.
Joosten, V.
Hovenkamp, J.
Gouka, R.J.
Verrips, C.T.
van den Hondel, C.A.M.J.J.
Publication year
2003
Abstract
The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP. © 2003 Elsevier Science B.V. All rights reserved.
Subject
Biotechnology
Aspergillus awamori
Heme limitation
Heterologous protein production
Peroxidase
Cell culture
Enzyme kinetics
Fungi
Growth kinetics
Molecular weight
Western blot analysis
Biotechnology
fungal enzyme
peroxidase
xylan endo 1,3 beta xylosidase
arthromyces ramosus
article
Aspergillus awamori
culture medium
enzyme analysis
enzyme synthesis
fungus
molecular weight
nonhuman
priority journal
Western blotting
Aspergillus
Biotechnology
Blotting, Northern
Blotting, Southern
Fermentation
Heme
Mitosporic Fungi
Peroxidase
Plasmids
Promoter Regions (Genetics)
'Arthromyces ramosus'
Aspergillus
Aspergillus awamori
Fungi
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DOI
https://doi.org/10.1016/s0168-1656(03)00109-3
TNO identifier
237151
ISSN
0168-1656
Source
Journal of Biotechnology, 103 (2), 183-190
Document type
article