Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori
van den Hondel, C.A.M.J.J.
The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP. © 2003 Elsevier Science B.V. All rights reserved.
To reference this document use:
Heterologous protein production
Western blot analysis
xylan endo 1,3 beta xylosidase
Promoter Regions (Genetics)
Journal of Biotechnology, 103 (2), 183-190