Title
Respiratory irritation by trimellitic anhydride in Brown Norway and Wistar rats
Author
Arts, J.H.E.
de Koning, M.W.
Bloksma, N.
Kuper, C.F.
TNO Voeding
Publication year
2001
Abstract
Several acid anhydrides are known for their sensitizing and irritative properties. Since both irritation and respiratory allergy can cause changes of lung function, proper testing of allergen-dependent effects on the respiratory tract requires knowledge of the respiratory irritant effects. To study the latter effects, groups of female Brown Norway (BN) and Wistar rats were exposed for 30 min to a range of concentrations (10 to 300 mg/m3) of the well-known respiratory allergen trimellitic anhydride (TMA). Breathing pattern and frequency were monitored before, during, and after exposure. Animals were necropsied and lung weights were determined 1 day after exposure. In BN rats, changes in breathing pattern were seen at levels of 29 mg/m3 and higher and decreases in frequency at 60 mg/m3 and higher, whereas in Wistar rats changes in both pattern and frequency (increases followed by decreases) were seen at levels of 34 mg/m3 and higher. Changes in breathing pattern consisted of a spiked form instead of a wave form of the respiratory cycle, with a pause between breaths at the end of expiration. The length of the pause increased with increasing concentrations of TMA while the duration of the respiratory cycle decreased slightly, implying that breathing frequency was mainly determined by the magnitude of the increase in pause. These reversible changes in breathing pattern and frequency were considered to be suggestive of lower airway irritation, rather than upper airway irritation. No concentration-related changes in lung weights were observed. The highest level at which no acute airway irritation as based on both breathing pattern and frequency was observed in both rat strains was 14 mg/m3.
Subject
trimellitic anhydride
animal experiment
article
breathing pattern
concentration response
controlled study
female
lung function
lung weight
nonhuman
priority journal
rat
rat strain
respiratory tract allergy
statistical analysis
Administration, Inhalation
Algorithms
Anhydrides
Animals
Female
Immunoglobulin E
Irritants
Lung
Metals
Organ Size
Particle Size
Rats
Rats, Inbred BN
Rats, Wistar
Respiratory Function Tests
Respiratory Tract Diseases
Animalia
Rattus norvegicus
To reference this document use:
http://resolver.tudelft.nl/uuid:3082acaa-9952-4884-9854-ee77941d8c17
TNO identifier
236225
ISSN
0895-8378
Source
Inhalation Toxicology, 13 (8), 719-728
Document type
article