Print Email Facebook Twitter Increased infiltration of Chlamydophila pneumoniae in the vessel wall of human veins after perfusion Title Increased infiltration of Chlamydophila pneumoniae in the vessel wall of human veins after perfusion Author Kupreishvili, K. ter Weeme, M. Morré, S.A. van den Brule, A.J.C. Huybregts, M.A.J.M. Quax, P.H.A. ten Velden, J. van Hinsbergh, V.W.M. Stooker, W. Eijsman, L. Niessen, H.W.M. TNO Kwaliteit van Leven Publication year 2008 Abstract Background: Several studies have suggested an association between Chlamydophila pneumoniae (Cp) infection and atherosclerosis. A recent study detected Cp DNA in the saphenous vein of 12% of all patients before bypass grafting and in 38% of failed grafts. We used a system in which human veins were perfused with autologous blood under arterial pressure. Materials and methods: Veins were surplus segments of saphenous veins of coronary artery bypass grafting (CABG) patients. Vein grafts were perfused with the blood of the same patient after CABG procedures. Veins were analysed for Cp-specific membrane protein using immunohistochemical and PCR analysis. Veins were analysed before and after perfusion (up to 4 h). The number of Cp positive cells was then quantified in the vein layers. Results: Cp protein was detected within macrophages only. In non-perfused veins, Cp was present in the adventitia in 91% of all patients, in the circular (64%) and longitudinal (23%) layer of the media. No positivity was found in the intima. Perfusion subsequently resulted in a significant increase of Cp positive cells within the circular layer of the media that, however, differed strongly between different patients. Cp DNA was not detected by PCR in those specimens. Conclusion: Cp protein was present in 91% of veins, but the number of positive cells differed remarkably between patients. Perfusion of veins resulted in increased infiltration of Cp into the circular layer. These results may point to a putative discriminating role of Cp with respect to graft failure between different patients. © 2008 The Authors. Subject HealthChlamydophila pneumoniae (Cp)Coronary artery bypass graft (CABG)Saphenous veinDNAmembrane proteinadventitiaarterial pressurearticleblood autotransfusionblood vessel wallbypass surgerycell infiltrationChlamydophila pneumoniaecoronary artery bypass grafthumanhuman tissueimmunohistochemistrymacrophageperfusionpolymerase chain reactionpriority journalsaphenous veinveinvein graftChlamydia InfectionsChlamydophila pneumoniaeCoronary Artery BypassCoronary Artery DiseaseDNA, BacterialHumansModels, BiologicalPerfusionPolymerase Chain ReactionSaphenous VeinStatistics as Topic To reference this document use: http://resolver.tudelft.nl/uuid:30700c7e-d3d1-48c0-86fc-a31f0b5a577e DOI https://doi.org/10.1111/j.1365-2362.2008.01961.x TNO identifier 240895 ISSN 0014-2972 Source European Journal of Clinical Investigation, 38 (7), 462-468 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.