Title
Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry
Author
Majchrzykiewicz-Koehorst, J.A.
Heikens, E.
Trip, H.
Hulst, A.G.
de Jong, A.L.
Viveen, M.C.
Sedee, N.J.A.
van der Plas, J.
Coenjaerts, F.E.J.
Paauw, A.
Publication year
2015
Abstract
The rapid identification of existing and emerging respiratory viruses is crucial in combating outbreaks and epidemics. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and reliable identification method in bacterial diagnostics, but has not been used in virological diagnostics. Mass spectrometry systems have been investigated for the identification of respiratory viruses. However, sample preparation methods were laborious and time-consuming. In this study, a reliable and rapid sample preparation method was developed allowing identification of cultured respiratory viruses. Tenfold serial dilutions of ten cultures influenza A strains, mixed samples of influenza A virus with human metapneumovirus or respiratory syncytial virus, and reconstituted clinical samples were treated with the developed sample preparation method. Subsequently, peptides were subjected to MALDI-TOF MS and liquid chromatography tandem mass spectrometry (LC-MS/MS). The influenza A strains were identified to the subtype level within 3. h with MALDI-TOF MS and 6. h with LC-MS/MS, excluding the culturing time. The sensitivity of LC-MS/MS was higher compared to MALDI-TOF MS. In addition, LC-MS/MS was able to discriminate between two viruses in mixed samples and was able to identify virus from reconstituted clinical samples. The development of an improved and rapid sample preparation method allowed generic and rapid identification of cultured respiratory viruses by mass spectrometry.
Subject
Life
CBRN - CBRN Protection
TS - Technical Sciences
Biology
Identification
Influenza virus
LC-MS/MS
MALDI-TOF MS
Mass spectrometry
Virological diagnosis
virus protein
Amino acid sequence
Controlled study
Influenza virus A
Intermethod comparison
Liquid chromatography
Matrix assisted laser desorption ionization time of flight mass spectrometry
Metapneumovirus
Nonhuman
Nucleotide sequence
Process development
Reliability
Respiratory syncytial pneumovirus
Respiratory virus
RNA sequence
Sensitivity analysis
Sequence analysis
Tandem mass spectrometry
Virus identification
Virus strain
Bacteria (microorganisms)
Human metapneumovirus
Influenza A virus
Orthomyxoviridae
Respiratory syncytial virus
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DOI
https://doi.org/10.1016/j.jviromet.2014.11.014
TNO identifier
521644
Publisher
Elsevier
ISSN
0166-0934
Source
Journal of Virological Methods, 213, 75-83
Document type
article