Print Email Facebook Twitter Effect of folate-binding protein on intestinal transport of folic acid and 5-methyltetrahydrofolate across Caco-2 cells Title Effect of folate-binding protein on intestinal transport of folic acid and 5-methyltetrahydrofolate across Caco-2 cells Author Verwei, M. van den Berg, H. Havenaar, R. Groten, J.P. TNO Defensie en Veiligheid TNO Kwaliteit van Leven Publication year 2005 Abstract Background: Milk products are a potential matrix for fortification with synthetic folic acid or natural 5-methyltetrahydrofolate (5-CH 3-H4folate) to enhance the daily folate intake. In milk, folate occurs bound to folatebinding proteins (FBP). Our previous studies with an in vitro gastrointestinal model showed that 70% of the initial FBP content of the milk product was retained in the duodenal lumen. While folic acid remained bound to FBP after gastric passage, 5-CH3-H4folate was mainly present as free folate in the duodenal lumen. Aim of the study: To investigate the effect of FBP on the absorption of folic acid and 5-CH 3-H4folate from the intestinal lumen. Methods: The transport of [3H]-folic acid and [14C]-5-CH 3-H4folate across enterocytes was studied in the presence or absence of bovine FBP using monolayers of Caco-2 cells grown on semi-permeable inserts in a two-compartment model. The apparent permeability coefficients (Papp) of folic acid and 5-CH3-H 4folate were determined and compared with the permeability of reference compounds for low (mannitol) and high (caffeine) permeability. Results: The transport from the apical to the basolateral side of the Caco-2 cells was higher (P < 0.05) for folic acid (Papp = 1.7*10-6 cm/s) than for 5- CH3-H4folate (Papp = 1.4*10-6 cm/s) after 2 h incubation to 1 μM folic acid or 5-CH3-H4folate test solutions (pH 7). The permeability of folic acid and 5-CH3-H4folate across Caco-2 monolayers appeared to be higher (P < 0.05) than that of mannitol (Papp = 0.5*10-6 cm/s) but lower (P < 0.05) than that of caffeine (Papp = 34*10-6 cm/s). The addition of FBP to the medium led to a lower (P < 0.05) intestinal transport and cellular accumulation of folic acid and 5-CH3-H4folate. Conclusions: Compared to the reference compounds, folic acid and 5-CH 3-H4folate showed a moderate permeability across Caco-2 cells, which indicates that folate absorption from the intestinal lumen is not likely to be complete. The intestinal transport of folic acid and 5-CH 3-H4folate was found to be dependent on the extent of binding to FBP at the luminal side of the cells. © Steinkopff Verlag 2004. Subject BiologyBiomedical ResearchBioavailabilityCaco-2FolateFolate-binding proteinIntestinal transport5 methyltetrahydrofolic acidcaffeinefolate binding proteinfolic acidmannitolarticlecell growthcell membrane permeabilitycontrolled studyhumanhuman cellintestine absorptionintestine cellmonolayer cultureprotein bindingBiological TransportCaco-2 CellsCarrier ProteinsCell Culture TechniquesChromatography, GelFolic AcidHumansIntestinal AbsorptionIntestine, SmallReceptors, Cell SurfaceTetrahydrofolatesTime FactorsBovinae To reference this document use: http://resolver.tudelft.nl/uuid:2c413eb6-524d-4a7f-b041-7da2d294434e DOI https://doi.org/10.1007/s00394-004-0516-9 TNO identifier 238514 ISSN 1436-6207 Source European Journal of Nutrition, 44 (4), 242-249 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.