Print Email Facebook Twitter The association of 83 plasma proteins with CHD mortality, BMI, HDL-, and total-cholesterol in men: Applying multivariate statistics to identify proteins with prognostic value and biological relevance Title The association of 83 plasma proteins with CHD mortality, BMI, HDL-, and total-cholesterol in men: Applying multivariate statistics to identify proteins with prognostic value and biological relevance Author Geert Heidema, A. Thissen, U. Boer, J.M.A. Bouwman, F.G. Feskens, E.J.M. Mariman, E.C.M. TNO Kwaliteit van Leven Publication year 2009 Abstract In this study, we applied the multivariate statistical tool Partial Least Squares (PLS) to analyze the relative importance of 83 plasma proteins in relation to coronary heart disease (CHD) mortality and the intermediate end points body mass index, HDL-cholesterol and total cholesterol. From a Dutch monitoring project for cardiovascular disease risk factors, men who died of CHD between initial participation (1987-1991) and end of follow-up (January 1, 2000) (N = 44) and matched controls (N = 44) were selected. Baseline plasma concentrations of proteins were measured by a multiplex immunoassay. With the use of PLS, we identified 15 proteins with prognostic value for CHD mortality and sets of proteins associated with the intermediate end points. Subsequently, sets of proteins and intermediate end points were analyzed together by Principal Components Analysis, indicating that proteins involved in inflammation explained most of the variance, followed by proteins involved in metabolism and proteins associated with total-C. This study is one of the first in which the association of a large number of plasma proteins with CHD mortality and intermediate end points is investigated by applying multivariate statistics, providing insight in the relationships among proteins, intermediate end points and CHD mortality, and a set of proteins with prognostic value. © 2009 American Chemical Society. Subject Biology Health NutritionAnalytical researchBMICHDHDL-cholesterolPartial least squaresProteomicsalpha 2 macroglobulinapolipoprotein A1beta 2 microglobulinC reactive proteinCD40 antigencholesterolfibrinogengrowth hormonehigh density lipoprotein cholesterolimmunoglobulin Minsulinleptinmacrophage derived chemokinemacrophage inflammatory protein 1betamatrix metalloproteinaseplasma proteinplasminogen activator inhibitor 1pregnancy associated plasma protein Atissue inhibitor of metalloproteinase 1vascular cell adhesion molecule 1vasculotropinadultangiogenesisarticleblood clottingbody masscardiovascular diseasecase control studycholesterol blood levelcholesterol metabolismclinical articlecontrolled studydisease associationenzyme activityfollow uphumanimmune responseimmunoassayinflammationischemic heart diseaselipid metabolismmalemortalitymultivariate analysisNetherlandspartial least squares regressionprincipal component analysispriority journalprognosisprotein analysisproteomicsrisk factorstatistical analysisunivariate analysis To reference this document use: http://resolver.tudelft.nl/uuid:2b6469c5-ca73-4393-8b21-8f6e30b96b30 DOI https://doi.org/10.1021/pr8006182 TNO identifier 241591 ISSN 1535-3893 Source Journal of Proteome Research, 8 (6), 2640-2649 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.