Title
The association of 83 plasma proteins with CHD mortality, BMI, HDL-, and total-cholesterol in men: Applying multivariate statistics to identify proteins with prognostic value and biological relevance
Author
Geert Heidema, A.
Thissen, U.
Boer, J.M.A.
Bouwman, F.G.
Feskens, E.J.M.
Mariman, E.C.M.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
In this study, we applied the multivariate statistical tool Partial Least Squares (PLS) to analyze the relative importance of 83 plasma proteins in relation to coronary heart disease (CHD) mortality and the intermediate end points body mass index, HDL-cholesterol and total cholesterol. From a Dutch monitoring project for cardiovascular disease risk factors, men who died of CHD between initial participation (1987-1991) and end of follow-up (January 1, 2000) (N = 44) and matched controls (N = 44) were selected. Baseline plasma concentrations of proteins were measured by a multiplex immunoassay. With the use of PLS, we identified 15 proteins with prognostic value for CHD mortality and sets of proteins associated with the intermediate end points. Subsequently, sets of proteins and intermediate end points were analyzed together by Principal Components Analysis, indicating that proteins involved in inflammation explained most of the variance, followed by proteins involved in metabolism and proteins associated with total-C. This study is one of the first in which the association of a large number of plasma proteins with CHD mortality and intermediate end points is investigated by applying multivariate statistics, providing insight in the relationships among proteins, intermediate end points and CHD mortality, and a set of proteins with prognostic value. © 2009 American Chemical Society.
Subject
Biology Health Nutrition
Analytical research
BMI
CHD
HDL-cholesterol
Partial least squares
Proteomics
alpha 2 macroglobulin
apolipoprotein A1
beta 2 microglobulin
C reactive protein
CD40 antigen
cholesterol
fibrinogen
growth hormone
high density lipoprotein cholesterol
immunoglobulin M
insulin
leptin
macrophage derived chemokine
macrophage inflammatory protein 1beta
matrix metalloproteinase
plasma protein
plasminogen activator inhibitor 1
pregnancy associated plasma protein A
tissue inhibitor of metalloproteinase 1
vascular cell adhesion molecule 1
vasculotropin
adult
angiogenesis
article
blood clotting
body mass
cardiovascular disease
case control study
cholesterol blood level
cholesterol metabolism
clinical article
controlled study
disease association
enzyme activity
follow up
human
immune response
immunoassay
inflammation
ischemic heart disease
lipid metabolism
male
mortality
multivariate analysis
Netherlands
partial least squares regression
principal component analysis
priority journal
prognosis
protein analysis
proteomics
risk factor
statistical analysis
univariate analysis
To reference this document use:
http://resolver.tudelft.nl/uuid:2b6469c5-ca73-4393-8b21-8f6e30b96b30
DOI
https://doi.org/10.1021/pr8006182
TNO identifier
241591
ISSN
1535-3893
Source
Journal of Proteome Research, 8 (6), 2640-2649
Document type
article