Title
Association between thrombin activatable fibrinolysis inhibitor genotype and levels in plasma: Comparison of different assays
Author
Guimarães, A.H.C.
van Tilburg, N.H.
Vos, H.L.
Bertina, R.M.
Rijken, D.C.
Publication year
2004
Abstract
Thrombin activatable fibrinolysis inhibitor (TAFI) antigen levels exhibit a large interindividual variability in which genetic control seems to play a major role. However, recent reports have questioned the association between TAFI concentration and genotype, suggesting that variable antibody reactivity towards TAFI isoforms, particularly the Thr325Ile polymorphism (1040C/T), may lead to artefacts in TAFI antigen levels. In order to compare assay outcome we determined plasma TAFI levels in 92 healthy individuals, using an enzyme-linked immunosorbent assay (ELISA) (commercial antibodies), an electroimmunoassay (in-house antibodies) and a commercial chromogenic assay (Actichrome® TAFI). Each individual was genotyped for the -438A/G and 1040C/T polymorphisms in the TAFI gene. TAFI levels were significantly associated with genotype in both antigen and chromogenic assays. All assays displayed significant correlations with each other. Linear regression and Bland-Altman agreement analysis in the genotype subgroups showed that neither the genotype nor the concentration affected the relationship between the Actichrome® TAFI and the electroimmunoassay. In contrast, the ELISA/Actichrome® TAFI and the ELISA/electroimmunoassay relationships were concentration- and genotype-dependent. Our results demonstrate that artefacts may arise when measuring TAFI antigen levels by ELISA. Nevertheless, the electroimmunoassay and the Actichrome® TAFI assay support a genotype-related variation of TAFI concentration. © 2004 Blackwell Publishing Ltd. Chemicals / CAS: Carboxypeptidase U, EC 3.4.17.20
Subject
Biomedical Research
Assay methods
Fibrinolysis
Genetic polymorphisms
Healthy individuals
Thrombin activatable fibrinolysis inhibitor
controlled study
correlation analysis
DNA polymorphism
Electroimmunoassay
Enzyme analysis
Enzyme assay
Enzyme blood level
Enzyme linked immunosorbent assay
Intermethod comparison
Linear regression analysis
Adult
Aged
Carboxypeptidase U
Female
Genotype
Humans
Immunoassay
Male
Middle Aged
Polymorphism, Genetic
Regression Analysis
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DOI
https://doi.org/10.1111/j.1365-2141.2004.04824.x
TNO identifier
237657
ISSN
0007-1048
Source
British Journal of Haematology, 124 (5), 659-665
Document type
article