Title
One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein
Author
Smit, E.
Pouwels, P.H.
Publication year
2002
Abstract
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and sufficient for cell wall binding. Our data suggest that SAC anchors the S-protein to a cell wall teichoic acid. Chemicals/CAS: Bacterial Proteins; Disinfectants; Membrane Glycoproteins; Membrane Proteins; Phenol, 108-95-2; surface array protein, bacteria
Subject
Cell membrane protein
Sac1 protein
Unclassified drug
Vitronectin
Amino terminal sequence
Bacterial cell wall
Bacterial genetics
Bacterium adherence
Carboxy terminal sequence
Controlled study
Nonhuman
Protein domain
Bacterial Proteins
Cell Wall
Disinfectants
Lactobacillus acidophilus
Membrane Glycoproteins
Membrane Proteins
Phenol
Protein Binding
Protein Structure, Tertiary
Sonication
Actinobacteria (class)
Bacteria (microorganisms)
Lactobacillus
Lactobacillus acidophilus
Uncultured actinomycete
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DOI
https://doi.org/10.1128/jb.184.16.4617-4619.2002
TNO identifier
236651
ISSN
0021-9193
Source
Journal of Bacteriology, 184 (184), 4617-4619
Document type
article