Print Email Facebook Twitter Validation of transferability of DBA derivatization and LC-MS/MS determination method for isocyanates via an interlaboratory comparison Title Validation of transferability of DBA derivatization and LC-MS/MS determination method for isocyanates via an interlaboratory comparison Author Bobeldijk, I. Karlsson, D. Pronk, A. Gonsalves, J. Hekman, M. van de Lagemaat, D. Preller, L. Heederik, D. Skarping, G. TNO Kwaliteit van Leven Publication year 2008 Abstract An adapted method for the quantitative determination of isocyanates in air was implemented and validated in-house. The method was based on air sampling using an impinger flask containing di-n-butylamine (DBA) in toluene and a glass fibre filter in series. The DBA derivatives were determined using liquid chromatography and tandem mass spectrometry. Studied isocyanates were isophorone diisocyanate, isocyanic acid (ICA), methyl isocyanate, ethyl isocyanate, propyl isocyanate, hexamethylene diisocyanate (HDI), 2,6- and 2,4-toluene diisocyanate, 4,4′-methylene diphenyl diisocyanate (MDI), phenyl isocyanate (PhI), MDI oligomers and different HDI adducts. Monitoring of selected reactions resulted in quantifications with correlation coefficients >0.995, within-batch relative standard deviation (RSD) of repeatability was <13% for all analytes. Between-batch RSD (reproducibility) was determined for all the compounds with the exception of the adducts and oligomers and was also <13%. As an additional validation procedure, the method was evaluated by exchanging field (air) and standard samples between two laboratories. The RSDs observed by the two laboratories were comparable. The concentrations determined were between 80 and 120% of each other, depending on the analyte and the individual concentrations. The method was applied in a large field study on exposure of workers in car repair shops and industrial painters with >500 samples. © The Author 2008. Published by Oxford University Press on behalf of the British Occupational Hygiene Society. Subject Analytical researchDBAInterlaboratory method comparisonIsocyanatesAdapted methodsAir samplingsAnalyteAnalytesCar repairsCorrelation coefficientsDBADerivatizationEthyl isocyanatesField studiesGlass fibresHexamethylene diisocyanateInter-laboratory comparisonsInterlaboratory method comparisonIsocyanatesIsocyanic acidsIsophorone diisocyanateLc-ms/msLiquid chromatography mass spectrometryMethyl isocyanatesMethyl-enediphenyl-diisocyanaten-ButylaminePhenyl isocyanatesQuantitative determinationsRelative standard deviationsReproducibilityStandard samplesTandem mass spectrometriesToluene diisocyanateAir filtersBody fluidsChromatographic analysisChromatographyFrequency allocationFullerenesGlass fibersHigh performance liquid chromatographyMass spectrometersMass spectrometryNonvolatile storagePolymersSemiconductor switchesSpectrometersSpectrometrySpectrum analysisTolueneLiquid chromatography2,6 toluene diisocyanate4,4' methylene diphenyl diisocyanateButylamineEthyl isocyanateHexamethylene diisocyanateIsocyanateIsocyanic acid derivativeIsophorone diisocyanateMethyl isocyanateOligomerPhenyl isocyanatePropyl isocyanateToluene diisocyanateUnclassified drugAir samplingComparative studyConcentration (parameters)Controlled studyLiquid chromatographyQuantitative assayReproducibility To reference this document use: http://resolver.tudelft.nl/uuid:2705dd1e-d1fd-4136-a16c-a7b93072aaf3 DOI https://doi.org/10.1093/annhyg/men057 TNO identifier 241097 ISSN 0003-4878 Source Annals of Occupational Hygiene, 52 (8), 757-763 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.