Title
Fungal protein production: Design and production of chimeric proteins
Author
Punt, P.J.
Levasseur, A.
Visser, H.
Wery, J.
Record, E.
Publication year
2011
Abstract
For more than a century, filamentous fungi have been used for the production of a wide variety of endogenous enzymes of industrial interest. More recently, with the use of genetic engineering tools developed for these organisms, this use has expanded for the production of nonnative heterologous proteins. In this review, an overview is given of examples describing the production of a special class of these proteins, namely chimeric proteins. The production of two types of chimeric proteins have been explored: (a) proteins grafted for a specific substrate-binding domain and (b) fusion proteins containing two separate enzymatic activities. Various application areas for the use of these chimeric proteins are described. © 2011 by Annual Reviews. All rights reserved.
Subject
Life
MSB - Microbiology and Systems Biology
EELS - Earth, Environmental and Life Sciences
Nutrition
Aspergillus
Carbohydrate-binding module
CAZY
Chrysosporium
Fusion protein
Purification
Trichoderma
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DOI
https://doi.org/10.1146/annurev.micro.112408.134009
TNO identifier
436594
ISSN
0066-4227
Source
Annual Review of Microbiology, 65, 57-69
Document type
bookPart