Print Email Facebook Twitter Determination of kava lactones in food supplements by liquid chromatography-atmospheric pressure chemical ionisation tandem mass spectrometry Title Determination of kava lactones in food supplements by liquid chromatography-atmospheric pressure chemical ionisation tandem mass spectrometry Author Bobeldijk, I. Boonzaaijer, G. Spies-Faber, E.J. Vaes, W.H.J. TNO Kwaliteit van Leven Publication year 2005 Abstract Reversed-phase liquid chromatography and detection with atmospheric pressure chemical ionisation tandem mass spectrometry was used for the determination of kava extracts in herbal mixtures. One percent of kava extract can be detected, corresponding to approximately 0.05-0.2 mg/g of the individual kava lactones kavain, dihydrokavain, yangonin, desmethoxyyangonin, methysticin and dihydromethysticin. Reliable quantification is obtained from concentrations of 0.25-1 mg/g, depending on the compound. At these concentration levels, the relative standard deviations were 10-14%. Validation showed good linearity and recoveries for all the kava lactones with the exception of yangonin. During method development, degradation of yangonin was observed. The degradation product was identified by nuclear magnetic resonance (NMR) as cis-yangonin. The method was applied to the analysis of commercial herbal products available in the Dutch market before and after market restrictions of kava-containing preparations. The results showed that even though 'old' products contained kava extract, the new formulations were negative on kava lactones. cis-Yangonin was also present in the herbal products. © 2005 Elsevier B.V. All rights reserved. Subject BiologyAnalytical researchKava lactonesLiquid chromatographyPiper methysticumTandem mass spectrometryAtmospheric pressureDrug productsIonizationLiquid chromatographyMass spectrometryNuclear magnetic resonanceSugarsFood supplementsHerbal mixturesHerbal productsKava extractsKetonesdesmethoxyyangonindihydrokawaindihydromethysticinherbaceous agentkava extractkawainlactonemethysticinunclassified drugyangoninarticlecis isomerdiet supplementationdrug degradationdrug determinationmass spectrometrynuclear magnetic resonance imagingpriority journalreversed phase liquid chromatographyvalidation processAtmospheric PressureCalibrationChromatography, LiquidFood AnalysisKavaMagnetic Resonance SpectroscopyMass SpectrometryReference StandardsReproducibility of ResultsPiper methysticum To reference this document use: http://resolver.tudelft.nl/uuid:259ac774-033d-4eef-8172-766badc2fe93 DOI https://doi.org/10.1016/j.chroma.2005.01.013 TNO identifier 238388 ISSN 0021-9673 Source Journal of Chromatography A, 1067 (1-2), 107-114 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.