Title
Determination of kava lactones in food supplements by liquid chromatography-atmospheric pressure chemical ionisation tandem mass spectrometry
Author
Bobeldijk, I.
Boonzaaijer, G.
Spies-Faber, E.J.
Vaes, W.H.J.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Reversed-phase liquid chromatography and detection with atmospheric pressure chemical ionisation tandem mass spectrometry was used for the determination of kava extracts in herbal mixtures. One percent of kava extract can be detected, corresponding to approximately 0.05-0.2 mg/g of the individual kava lactones kavain, dihydrokavain, yangonin, desmethoxyyangonin, methysticin and dihydromethysticin. Reliable quantification is obtained from concentrations of 0.25-1 mg/g, depending on the compound. At these concentration levels, the relative standard deviations were 10-14%. Validation showed good linearity and recoveries for all the kava lactones with the exception of yangonin. During method development, degradation of yangonin was observed. The degradation product was identified by nuclear magnetic resonance (NMR) as cis-yangonin. The method was applied to the analysis of commercial herbal products available in the Dutch market before and after market restrictions of kava-containing preparations. The results showed that even though 'old' products contained kava extract, the new formulations were negative on kava lactones. cis-Yangonin was also present in the herbal products. © 2005 Elsevier B.V. All rights reserved.
Subject
Biology
Analytical research
Kava lactones
Liquid chromatography
Piper methysticum
Tandem mass spectrometry
Atmospheric pressure
Drug products
Ionization
Liquid chromatography
Mass spectrometry
Nuclear magnetic resonance
Sugars
Food supplements
Herbal mixtures
Herbal products
Kava extracts
Ketones
desmethoxyyangonin
dihydrokawain
dihydromethysticin
herbaceous agent
kava extract
kawain
lactone
methysticin
unclassified drug
yangonin
article
cis isomer
diet supplementation
drug degradation
drug determination
mass spectrometry
nuclear magnetic resonance imaging
priority journal
reversed phase liquid chromatography
validation process
Atmospheric Pressure
Calibration
Chromatography, Liquid
Food Analysis
Kava
Magnetic Resonance Spectroscopy
Mass Spectrometry
Reference Standards
Reproducibility of Results
Piper methysticum
To reference this document use:
http://resolver.tudelft.nl/uuid:259ac774-033d-4eef-8172-766badc2fe93
DOI
https://doi.org/10.1016/j.chroma.2005.01.013
TNO identifier
238388
ISSN
0021-9673
Source
Journal of Chromatography A, 1067 (1-2), 107-114
Document type
article