Title
To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies
Author
Aguirre, M.
Ramiro-Garcia, J.
Koenen, M.E.
Venema, K.
Publication year
2014
Abstract
This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments. Chemicals/CAS: isobutyric acid, 5711-69-3, 79-31-2; isovaleric acid, 503-74-2, 539-66-2
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Fecal inoculum preparation
Fermentation
Gut microbiota
In vitro model
Pooling
Branched chain fatty acid
Fatty acid
Isobutyric acid
Isovaleric acid
Short chain fatty acid
Unclassified drug
Actinobacteria
Adult
Bacteroidetes
Clostridium
Controlled study
Feces
Female
Fermentation technique
Firmicutes
Genus
Human
Human experiment
In vitro study
Inoculation
Intestine flora
Microbial community
Nonhuman
Normal human
Prevotella
Proteobacteria
Sample
Turicibacter
Verrucomicrobia
To reference this document use:
http://resolver.tudelft.nl/uuid:2570268d-3fe9-44b7-99a3-26550848c348
DOI
https://doi.org/10.1016/j.mimet.2014.08.022
TNO identifier
517662
ISSN
0167-7012
Source
Journal of Microbiological Methods, 107, 1-7
Document type
article