Title
In-depth characterization of prebiotic galactooligosaccharides by a combination of analytical techniques
Author
Coulier, L.
Timmermans, J.
Richard, B.
van den Dool, R.
Haaksman, I.
Klarenbeek, B.
Slaghek, T.
van Dongen, W.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
A commercial prebiotic galacto-oligosaccharide mixture (Vivinal GOS) was extensively characterized using a combination of analytical techniques. The different techniques were integrated to give complementary information on specific characteristics of the oligosaccharide mixture, ranging from global information on degree of polymerization (DP) to the identity and concentration of individual oligosaccharides. The coupling of high-performance anion-exchange chromatography (HPAEC) to mass spectrometry (MS) was determined to be especially suitable to assign the DP of individual oligosaccharides on the basis of their m/z values as well as their quantification using external standards. The combination of NMR spectroscopy and methylation analysis after isolation using size exclusion chromatography (SEC) and hydrophilic interaction liquid chromatography (HILIC) was used for identification. All DP2 compounds could be identified and quantified in this way as well as the main DP3 compounds. ©2009 American Chemical Society.
Subject
Nutrition
Food technology
Analysis
Galacto-ollgosaccharldes
GC-MS
High-performance anlon-exchange chromatography
HILIC
Identification
Mass spectrometry
NMR
Prebiotics
Quantification
To reference this document use:
http://resolver.tudelft.nl/uuid:255c88d9-3cf6-4a0e-9aa8-8bd346be1cf0
DOI
https://doi.org/10.1021/jf902549e
TNO identifier
241973
ISSN
0021-8561
Source
Journal of Agricultural and Food Chemistry, 57 (18), 8488-8495
Document type
article