Title
Feasibility of a novel on-site detection method for aflatoxin in maize flour from markets and selected households in Kampala, Uganda
Author
Wacoo, A.P.
Wendiro, D.
Nanyonga, S.
Hawumba, J.F.
Sybesma, W.
Kort, R.
Publication year
2018
Abstract
In sub-Saharan Africa, there is a high demand for affordable and accessible methods for on-site detection of aflatoxins for appropriate food safety management. In this study, we validated an electrochemical immunosensor device by the on-site detection of 60 maize flour samples from six markets and 72 samples from households in Kampala. The immunosensor was successfully validated with a linear range from 0.7 ± 0.1 to 11 ± 0.3 µg/kg and limit of detection (LOD) of 0.7 µg/kg. The maize flour samples from the markets had a mean total aflatoxin concentration of 7.6 ± 2.3 µg/kg with approximately 20% of the samples higher than 10 µg/kg, which is the maximum acceptable level in East Africa. Further down the distribution chain, at the household level, approximately 45% of the total number contained total aflatoxin levels higher than the acceptable limit. The on-site detection method correlated well with the established laboratory-based HPLC and ELISA-detection methods for aflatoxin B1 with the correlation coefficients of 0.94 and 0.98, respectively. This study shows the feasibility of a novel on-site detection method and articulates the severity of aflatoxin contamination in Uganda. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Subject
Aflatoxins
ELISA
Households
HPLC
Immunosensor
Maize
Markets
To reference this document use:
http://resolver.tudelft.nl/uuid:24d84cfa-4680-4e69-995c-09a5300edd38
DOI
https://doi.org/10.3390/toxins10080327
TNO identifier
842619
ISSN
2072-6651
Source
Toxins, 10 (10)
Document type
article