Title
Genetic analysis of acidocin B: A novel bacteriocin produced by Lactobacillus acidophilus
Author
Leer, R.J.
van der Vossen, J.M.B.M.
van Giezen, M.
van Noort, J.M.
Pouwels, P.H.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1995
Abstract
The genes encoding the production of acidocin B, a bacteriocin produced by Lactobacillus acidophilus strain M46 which is active against Listeria monocytogenes, Clostridium sporogenes, Brochothrix thermosphacta, Lactobacillus fermentum and Lactobacillus delbrueckii subsp. bulgaricus, but inactive against most other Lactobacillus species, were previously localized on a 4 kb Xbal-HindIII fragment of plasmid pCV461. In the present work, DNA sequence analysis revealed the presence of three consecutive ORFs, which potentially code for hydrophobic peptides composed of 60, 91 and 114 amino acids, respectively, and a fourth ORF of opposite polarity which could potentially encode a peptide of 59 amino acids. The middle ORF (ORF-2; acdB) was identified as the gene encoding acidocin B by comparing the amino acid composition of highly purified acidocin B with the deduced amino acid sequence of ORF-2. Our results suggest that acidocin B is synthesized as a precursor which is processed at a site which conforms to the '-3, -1' rules of von Heijne to yield active acidocin B (59 amino acids). The presence of an immunity-protein-encoding gene on the 4 kb Xbal-BamHI fragment was deduced from the capacity of a plasmid vector harbouring this fragment to confer immunity upon transformation of L. fermentum NCK127. One of the three non-assigned ORFs may encode this immunity protein.
Subject
Biology
Amino Acid Sequence
Bacteriocins
Base Sequence
Chromatography, High Pressure Liquid
Cloning, Molecular
Clostridium
Conserved Sequence
DNA, Bacterial
Genes, Bacterial
Lactobacillus
Lactobacillus acidophilus
Molecular Sequence Data
Multigene Family
Open Reading Frames
Plasmids
Protein Sorting Signals
Restriction Mapping
Support, Non-U.S. Gov't
Transformation, Bacterial
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http://resolver.tudelft.nl/uuid:2376f3cf-8788-431e-9661-f95e2cd2d137
TNO identifier
37793
ISSN
1350-0872
Source
Microbiology, 141 (7), 1629-1635
Document type
article