Title
Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography-mass spectrometry
Author
Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding
van Puymbroeck, M.
Kuilman, M.E.M.
Maas, R.F.M.
Witkamp, R.F.
Leyssens, L.
van der Zande, D.J.M.
Gelan, J.
Raus, J.
Publication year
1998
Abstract
17alpha-Boldenone (17alpha-BOL) and/or 17beta-boldenone (17beta-BOL) appear occasionally in fecal matter of cattle. In addition to 17alpha-BOL, a whole array of boldenone related substances can be found in the same samples. In vitro experiments with microsomal liver preparations and isolated hepatocytes combined with the excretion profiles found in urine and feces samples of in vivo experiments made it possible to identify several metabolites of 17beta-BOL in 17beta-BOL positive feces samples. In one animal treated with 17beta-BOL, no 17beta-BOL or its metabolites were present before treatment and most of these compounds disappeared gradually in time after the treatment was stopped. It is not clear what the origin is of 17alpha-BOL and boldenone metabolites in samples screened routinely for the abuse of anabolic steroids and considered to be 'negative' because of the absence of 17beta-BOL since other workers showed some evidence that 17alpha-BOL can be of endogenous origin. However, in our hands, most of these 17alpha-BOL positive samples, obtained during routinely performed screenings of cattle contained large amounts of Δ4-androstene-3,17-dione (AED), which normally is absent from routinely screened negative samples. Furthermore, AED was absent in all samples obtained from the animals treated with 17beta-BOL. We have no direct evidence that 17alpha-BOL or 17beta-BOL is of endogenous origin.
Subject
Anabolic agent
Androstenedione
Boldenone
Drug metabolite
Animal tissue
Cattle
Conference paper
Drug feces level
Drug metabolism
Drug urine level
Gas chromatography
Liver cell
Liver microsome
Mass spectrometry
Nonhuman
Animals
Cells, Cultured
Feces
Gas Chromatography-Mass Spectrometry
Liver
Male
Microsomes, Liver
Testosterone
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http://resolver.tudelft.nl/uuid:230a944f-b329-4512-b0cf-c834efd8370c
DOI
https://doi.org/10.1039/a805003a
TNO identifier
234703
ISSN
0003-2654
Source
Analyst, 123 (123), 2681-2686
Document type
article