Print Email Facebook Twitter Molecular cloning and expression analysis of a monosaccharide transporter gene OsMST4 from rice (Oryza sativa L.) Title Molecular cloning and expression analysis of a monosaccharide transporter gene OsMST4 from rice (Oryza sativa L.) Author Wang, Y. Xu, H. Wei, X. Chai, C. Xiao, Y. Zhang, Y. Chen, B. Xiao, G. Ouwerkerk, P.B.F. Wang, M. Zhu, Z. TNO Kwaliteit van Leven Publication year 2007 Abstract Monosaccharide transporters mediate the membrane transport of a variable range of monosaccharides, which plays a crucial role in sugar distribution throughout the plant. To investigate the significance of monosaccharide transporters for rice (Oryza sativa L.) seed development, cDNA of a new putative monosaccharide transporter gene OsMST4 was isolated. The deduced OsMST4 protein shows typical features of monosaccharide transporters, and shares high homology with other plant homologues. Heterologous expression in yeast (Saccharomyces cerevisiae) showed that OsMST4 is a functional monosaccharide transporter capable of transporting glucose, fructose, mannose and galactose. Transcriptional analysis revealed that OsMST4 is expressed in all tested organs/tissues. In developing caryopses, its expression is high at the early and middle grain filling stages, and declines gradually to low levels after that. Further analysis revealed that it is expressed in both the maternal tissue and the filial tissue, with its highest expression in embryo. Cellular location in young caryopses through RNA in situ hybridization showed that OsMST4 mRNA mainly accumulates in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm. The expression pattern of OsMST4 was further confirmed by histochemical analysis of the OsMST4-promoter-β-glucuronidase (GUS) transgenic rice plants. These data indicate that OsMST4 is actively involved in monosaccharides supply for seed development during the course of grain filling. In addition, the cell type-specific expression patterns of OsMST4 in other sink and source tissues were also investigated, and its corresponding physiological roles were discussed. © 2007 Springer Science+Business Media B.V. Subject BiologyBiomedical ResearchcDNA cloningGene expressionMonosaccharide transporterOryza sativa L.Seed developmentCloningCropsFructoseGenesPhysiologyPlant cell cultureTranscriptioncDNA cloningMonosaccharide transporterOryza sativa L.Seed developmentMolecular biologycomplementary DNAglucose transportermessenger RNAprimer DNAvegetable proteinamino acid sequencearticlechemistrygene expression profilinggeneticsin situ hybridizationmolecular cloningmolecular geneticsnucleotide sequencephylogenyreverse transcription polymerase chain reactionricesequence homologyAmino Acid SequenceBase SequenceCloning, MolecularDNA PrimersDNA, ComplementaryGene Expression ProfilingIn Situ HybridizationMolecular Sequence DataMonosaccharide Transport ProteinsOryza sativaPhylogenyPlant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSequence Homology, Amino AcidOryza sativaSaccharomyces cerevisiae To reference this document use: http://resolver.tudelft.nl/uuid:2301a4ea-e573-4afb-a36f-ed486f1957f7 DOI https://doi.org/10.1007/s11103-007-9228-x TNO identifier 240257 ISSN 0167-4412 Source Plant Molecular Biology, 65 (4), 439-451 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.