Title
Detrimental effects of constant light exposure and high-fat diet on circadian energy metabolism and insulin sensitivity
Author
Coomans, C.P.
van den Berg, S.A.A.
Houben, T.
van Klinken, J.B.
van den Berg, R.
Pronk, A.C.M.
Havekes, L.M.
Romijn, J.A.
van Dijk, K.W.
Biermasz, N.R.
Meijer, J.H.
Publication year
2013
Abstract
Circadian rhythm disturbances are observed in, e.g., aging and neurodegenerative diseases and are associated with an increased incidence of obesity and diabetes. We subjected male C57Bl/6J mice to constant light [12-h light-light (LL) cycle] to examine the effects of a disturbed circadian rhythm on energy metabolism and insulin sensitivity. In vivo electrophysiological recordings in the central pacemaker of the suprachiasmatic nuclei (SCN) revealed an immediate reduction in rhythm amplitude, stabilizing at 44% of normal amplitude values after 4 d LL. Food intake was increased (+26%) and energy expenditure decreased (-13%), and we observed immediate body weight gain (d 4: +2.4%, d 14: +5.0%). Mixed model analysis revealed that weight gain developed more rapidly in response to LL as compared to high fat. After 4 wk in LL, the circadian pattern in feeding and energy expenditure was completely lost, despite continuing lowamplitude rhythms in the SCN and in behavior, whereas weight gain had stabilized. Hyperinsulinemic-euglycemic clamp analysis revealed complete abolishment of normal circadian variation in insulin sensitivity in LL. In conclusion, a reduction in amplitude of the SCN, to values previously observed in aged mice, is sufficient to induce a complete loss of circadian rhythms in energy metabolism and insulin sensitivity.
Subject
Life
MSB - Microbiology and Systems Biology MHR - Metabolic Health Research
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Health
Healthy Living
Constant light
Electrophysiology
Multiunit activity
SCN
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DOI
https://doi.org/10.1096/fj.12-210898
TNO identifier
471471
ISSN
1530-6860
Source
FASEB Journal, 27 (4), 1721-1732
Document type
article