Print Email Facebook Twitter Choline supplemented as phosphatidylcholine decreases fasting and postmethionine-loading plasma homocysteine concentrations in healthy men Title Choline supplemented as phosphatidylcholine decreases fasting and postmethionine-loading plasma homocysteine concentrations in healthy men Author Olthof, M.R. Brink, E.J. Katan, M.B. Verhoef, P. TNO Kwaliteit van Leven Publication year 2005 Abstract Background: A high homocysteine concentration is a potential risk factor for cardiovascular disease that can be reduced through betaine supplementation. Choline is the precursor for betaine, but the effects of choline supplementation on plasma total homocysteine (tHcy) concentrations in healthy humans are unknown. Objective: The objective was to investigate whether supplementation with phosphatidylcholine, the form in which choline occurs in foods, reduces fasting and postmethionine-loading concentrations of plasma tHcy in healthy men with mildly elevated plasma tHcy concentrations. Design: In a crossover study, 26 men ingested ≈2.6 g choline/d (as phosphatidylcholine) or a placebo oil mixture for 2 wk in random order. Fatty acid composition and fat content were similar for both treatments. A methionine-loading test was performed on the first and last days of each supplementation period. Results: Phosphatidylcholine supplementation for 2 wk decreased mean fasting plasma tHcy by 18% (-3.0 μmol/L; 95% CI: -3.9, -2.1 μmol/L). On the first day of supplementation, a single dose of phosphatidylcholine containing 1.5 g choline reduced the postmethionine-loading increase in tHcy by 15% (-4.8 μmol/L; 95% CI: -6.8, -2.8 μmol/L). Phosphatidylcholine supplementation for 2 wk reduced the postmethionine-loading increase in tHcy by 29% (-9.2 μmol/L; 95% CI: -11.3, -7.2 μmol/L). All changes were relative to placebo. Conclusions: A high daily dose of choline, supplemented as phosphatidylcholine, lowers fasting as well as postmethionine-loading plasma tHcy concentrations in healthy men with mildly elevated tHcy concentrations. If high homocysteine concentrations indeed cause cardiovascular disease, choline intake may reduce cardiovascular disease risk in humans. © 2005 American Society for Clinical Nutrition. Subject HealthBiomedical ResearchCholineHomocysteineHumansMethionine loadingPhosphatidylcholinecholinecyanocobalaminfatty acidfolic acidhomocysteinelipidmethioninephosphatidylcholinepyridoxineadultagedarticlebody weightclinical articleclinical trialcontrolled clinical trialcontrolled studydiet restrictionhumankidney function testliver function testloading testmalerandomized controlled trialbloodcross-sectional studydouble blind proceduredrug effectkidneylivermetabolismmiddle agedoral drug administrationAdministration, OralCholineCross-Sectional StudiesDouble-Blind MethodFastingHomocysteineHumansKidneyLiverMaleMethionineMiddle AgedPhosphatidylcholines To reference this document use: http://resolver.tudelft.nl/uuid:21d77678-ea5a-4cdc-bbba-9496c857e6b6 TNO identifier 239021 ISSN 0002-9165 Source American Journal of Clinical Nutrition, 82 (1), 111-117 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.