Print Email Facebook Twitter Human metabolomics: Strategies to understand biology Title Human metabolomics: Strategies to understand biology Author Ramautar, R. Berger, R. van der Greef, J. Hankemeier, T. Publication year 2013 Abstract Metabolomics provides a direct functional read-out of the physiological status of an organism and is in principle ideally suited to describe someone's health status. Whereas only a limited number of small metabolites are used in the clinics, in inborn errors of metabolism an extensive repertoire of metabolites are used as biomarkers. We discuss that the proper clinical phenotyping is crucial to find biomarkers and obtain biological insights for multifactorial diseases. This requires to study the phenotype dynamics including the concepts of homeostasis and allostasis, that is, the ability to adapt and cope with a challenge. We also elaborate that biology-driven metabolomics platforms (i.e. development of metabolomics technology driven by the need of studying and answering important biomedical questions) addressing clinically relevant pathways and at the same time providing absolute concentrations are key to allow discovery and validation of biomarkers across studies and labs. Following individuals over years will require high throughput metabolomics approaches, which are emerging for nuclear magnetic resonance spectroscopy and direct-infusion mass spectrometry, but should also include the biochemical networks needed for personalized health monitoring. © 2013 Elsevier Ltd. Chemicals/CAS: betaine, 107-43-7, 590-46-5; carnitine, 461-06-3, 541-15-1, 56-99-5; cholesterol, 57-88-5; choline, 123-41-1, 13232-47-8, 1927-06-6, 4858-96-2, 62-49-7, 67-48-1; creatinine, 19230-81-0, 60-27-5; glucose, 50-99-7, 84778-64-3; trimethylamine oxide, 1184-78-7; urea, 57-13-6 Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesFood and NutritionHealthHealthy LivingBetaineBiological markerCarnitineCholesterolCholineCreatinineGlucoseTrimethylamine oxideUreaAllostasisBiologyCapillary electrophoresisCardiovascular diseaseCardiovascular riskEnvironmental factorFatty acid oxidationGas chromatographyGenomicsHeredityHomeostasisHumanLimit of detectionLiquid chromatographyMass spectrometryMetabolomicsMolecular weightNon insulin dependent diabetes mellitusNonhumanNuclear magnetic resonance spectroscopyPhenotypeProteomicsSolvent extractionUtrafiltration To reference this document use: http://resolver.tudelft.nl/uuid:215e0b2e-338e-43e4-93a0-d9716d0fcc76 DOI https://doi.org/10.1016/j.cbpa.2013.06.015 TNO identifier 484272 ISSN 1367-5931 Source Current Opinion in Chemical Biology, 17 (5), 841-846 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.