Title
In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota
Author
Fehlbaum, S.
Prudence, K.
Kieboom, J.
Heerikhuisen, M.
van den Broek, T.
Schuren, F.H.J.
Steinert, R.E.
Raederstorff, D.
Publication year
2018
Abstract
Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli. Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied. An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition). All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers. Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production. Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition. A dose-response effect was observed for butyrate when exposed to beta-glucan and inulin. The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.
Subject
Healthy Living
Biomedical Innovation
XOS
Alpha-GOS
Beta-glucan
Colonic fermentation
I-screen
Screening platform
To reference this document use:
http://resolver.tudelft.nl/uuid:20ca01af-aca5-4a02-80d7-30b6010b9b3a
DOI
https://doi.org/10.3390/ijms19103097
TNO identifier
871914
Source
International Journal of Molecular Sciences, 19 (19), E3097
Document type
article