Title
The binding of sarin in the blood plasma of the rat
Author
Medisch Biologisch Labortorium TNO
Polak, R.L.
Cohen, E.M.
Publication year
1970
Abstract
Sephadex filtration of serum from rats 10 min after injection of 50 μg/kg of 32P-sarin demonstrated that about 70 per cent of the radioactivity present in the serum was attached to molecules with a molecular weight above 10,000. Incubation of samples of this serum with oximes caused a decrease in the amounts of 32P attached to the large molecules and an increase in those appearing in the micromolecular fractions. The oximes apparently released 32P from the large molecules. DAM and MINA were much more effective in this respect than P-2-AM and TMB-4. The activities of the ChE and AE in serum from rats 10 min after injection of sarin were determined. Both enzymes were partly inactivated. The AE reactivating potencies of the oximes concurred with their 32P releasing effects: DAM and MINA (0.5 mM) partly reactivated the AE, but P-2-AM and TMB-4 did not. In contrast TMB-4, P-2-AM and MINA produced a partial reactivation of the ChE whereas DAM had no effect. It was concluded that the obtained results support the idea that the large molecules in the serum to which the radioactivity was attached were identical with AE. © 1970.
Subject
Acetylcholinesterase
Cholinesterase
Cholinesterase inhibitor
Oxime
Phosphonic acid derivative
Phosphorus
Pralidoxime
Animal
Blood
Drug effect
Enzyme activation
Female
Gel chromatography
Intraperitoneal drug administration
Intravenous drug administration
Macromolecule
Metabolism
Molecular weight
Protein binding
Rat
Chromatography, Gel
Enzyme Activation
Injections, Intraperitoneal
Injections, Intravenous
Macromolecular Systems
Molecular Weight
Phosphonic Acids
Phosphorus Isotopes
Pralidoxime Compounds
Protein Binding
To reference this document use:
http://resolver.tudelft.nl/uuid:1fc129b5-1d5b-4abf-9130-2a28eb1089fa
TNO identifier
227160
ISSN
0006-2952
Source
Biochemical Pharmacology, 19 (19), 877-881
Document type
article