Title
Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon
Author
Martínez, B.
Fernández, M.
Suárez, J.E.
Rodríguez, A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
Synthesis of lactococcin 972 is plasmid-encoded. An operon composed of two genes that encode pre-bacteriocin and a putative immunity protein has been identified. The first gene encodes a 91-residue polypeptide that is exported via a sec-dependent system to give the mature 66-aa bacteriocin. The immunity protein is a 563-residue polypeptide with seven potential transmembrane domains. Two transcripts were observed from this region: one comprises the whole operon and is synthesized during the exponential phase of growth while the other, which corresponds just to the bacteriocin structural gene, presents a maximum in exponential cultures but is still present in late-stationary-phase cells.
Subject
Nutrition
Bacteriocin
Lactococcin 972
Lactococcus lactis
Plasmid-encoded genes
Lactococcin
Bacterial gene
Gene expression
Lactococcus lactis
Nonhuman
Nucleotide sequence
Operon
Plasmid
Priority journal
Protein synthesis
Structural gene
Amino Acid Sequence
Bacteriocins
Base Sequence
Blotting, Northern
Electrophoresis, Polyacrylamide Gel
Genes, Bacterial
Lactococcus lactis
Molecular Sequence Data
Operon
Plasmids
RNA, Bacterial
Time Factors
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TNO identifier
235201
ISSN
1350-0872
Source
Microbiology, 145 (11), 3155-3161
Document type
article