The progress of intestinal epithelial models from cell lines to gut-on-chip

The progress of intestinal epithelial models from cell lines to gut-on-chip

Rahman, S.
Ghiboud, M.
Donkers, J.M.
van de Steeg, E.
van Tol, E.A.F.
Hakvoort, T.B.M.
de Jonge, W.J.

2021

Over the past years, several preclinical in vitro and ex vivo models have been developed that helped to understand some of the critical aspects of intestinal functions in health and disease such as inflammatory bowel disease (IBD). However, the translation to the human in vivo situation remains problematic. The main reason for this is that these approaches fail to fully reflect the multifactorial and complex in vivo environment (e.g., including microbiota, nutrition, and immune response) in the gut system. Although conventional models such as cell lines, Ussing chamber, and the everted sac are still used, increasingly more sophisticated intestinal models have been developed over the past years including organoids, InTESTine™ and microfluidic gut-on-chip. In this review, we gathered the most recent insights on the setup, advantages, limitations, and future perspectives of most frequently used in vitro and ex vivo models to study intestinal physiology and functions in health and disease. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Ex vivo
Gut-on-chip
In vitro
Inflammation
Intestine
Organoids

http://resolver.tudelft.nl/uuid:1bfc523c-c910-4e42-b438-1729927621cd

https://doi.org/10.3390/ijms222413472

962202

International Journal of Molecular Sciences, 22 (22)

article