Title
Extracorporeal irradiation of the blood in a rat model for human acute myelocytic leukemia. Increased efficacy after combination with cell mobilization by low-molecular-weight dextran sulfate
Author
Hagenbeek, A.
Martens, A.C.M.
Radiobiologisch Instituut TNO
Publication year
1981
Abstract
The efficacy of extracorporeal irradiation of the blood (ECIB) in combination with cell mobilization by dextran sulfate (DS; MW 17,000) was investigated in a rat model for human acute myelocytic leukemia. Repeated injections with DS (q 3 hr) induced a significant increase in the number of peripheral leukemic cells, i.e., up to 4.5 times the original number 6 hr after the first injection. Cell mobilization in combination with ECIB (2 x 8 hr) caused a depletion of the blood compartments and the rapidly exchangeable tissue pool down to 10-25% of their original sizes, as determined by measuring the distribution of infused 51Cr-labeled leukemic cells and organ weights. These size reductions are about two times as great as those in rats treated with ECIB alone. In addition, the slowly exchangeable tissue pools are significantly depleted when DS is added to ECIB treatment. The reduction in the total tumor load was about 50%. This, however, is too small to result in a significant difference in survival time between treated and nontreated leukemic rats. Chemicals/CAS: chromium 51, 14392-02-0; dextran sulfate, 9011-18-1, 9042-14-2; Dextran Sulfate, 9042-14-2; Dextrans, 9004-54-0
Subject
Biology
chromium 51
Acute myeloblastic leukemia
Animal experiment
Biological model
Blood
Blood and hemopoietic system
Blood extracorporeal radiation
Irradiation
Animal
Blood
Cell Compartmentation
Dextran Sulfate
Dextrans
Human
Leukemia, Experimental
Leukemia, Myelocytic, Acute
Male
Rats
Rats, Inbred BN
To reference this document use:
http://resolver.tudelft.nl/uuid:18c5fdf9-8b87-485d-ace0-76a5704bc0be
TNO identifier
229046
ISSN
0033-7587
Source
Radiation Research, 88 (1), 144-154
Document type
article