Title
Effects of growth altitude on chemical constituents and delayed luminescence properties in medicinal rhubarb
Author
Sun, M.
Li, L.
Wang, M.
Van Wijk, E.
He, M.
van Wijk, R.
Koval, S.
Hankemeier, T.
van der Greef, J.
Wei, S.
Publication year
2016
Abstract
To improve the quality control of herbal drugs, there has been a major shift from evaluating individual chemicals to evaluating multiple-constituent chemicals, given the multi-pharmacology nature of herbal drugs. Therefore, rapid, systematic assays are needed in order to assess the quality of medicinal herbs using a comprehensive, integrated approach. Light-induced delayed luminescence (DL) is used to measure decaying long-term ultra-weak photon emissions following excitation with light. DL is considered to be a sensitive indicator for characterizing the properties of biological systems and herbal medicines with various therapeutic properties. The aim of this study was to examine the feasibility of using DL as a novel quality-assessment tool using rhubarb material as a model system, and to establish the correlation between DL parameters and the chemical constituents of rhubarb. Raw roots and rhizomes were collected from rhubarb (Rheum palmatum L.) at various elevations in western China. HPLC analysis was used to identify fourteen bioactive constituents. Five DL parameters were calculated from the DL decay curves of the rhubarb samples. Statistical tools, including principal component analysis, were used to classify the rhubarb samples using data obtained using two different assays. Finally, Spearman's correlation coefficient was calculated to quantify the correlation between the bioactive compounds and corresponding DL parameters. We found that both the chemical analysis and DL measurements reflect variations in the quality of rhubarb due to environment factor. The DL parameters were correlated significantly with the bioactive chemical constituents. Our results indicate that DL is a promising tool for evaluating multiple constituents and for assessing the therapeutic properties of herbal medicines. Thus, DL may be used as part of a comprehensive system for assessing the quality and/or therapeutic properties of herbal medicines. © 2016 Elsevier B.V. All rights reserved.
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Active constituents
Delayed luminescence
Environmental factor
Quality control
Rhubarb
To reference this document use:
http://resolver.tudelft.nl/uuid:183b925f-f92a-434d-bf09-24ee91d4d7a4
DOI
https://doi.org/10.1016/j.jphotobiol.2016.06.018
TNO identifier
537415
ISSN
1011-1344
Source
Journal of Photochemistry and Photobiology B: Biology, 162, 24-33
Document type
article