Print Email Facebook Twitter Pentosidine accumulates in the aging vitreous body: A gender effect Title Pentosidine accumulates in the aging vitreous body: A gender effect Author van Deemter, M. Ponsioen, T.L. Bank, R.A. Snabel, J.M.M. van der Worp, R.J. Hooymans, J.M.M. Los, L.I. TNO Kwaliteit van Leven Publication year 2009 Abstract The human vitreous body undergoes structural changes with aging. This can be followed by a posterior vitreous detachment, which can result in ocular pathology. As in many collagenous tissues, age-related changes in the vitreous could be caused by the formation of advanced glycation end products (AGEs). The goal of this study was to find out whether the AGE pentosidine accumulates in the human vitreous with aging. With this data we were able to estimate the half-life of vitreous collagen. Furthermore, we analyzed whether there was a gender difference in pentosidine accumulation, as this was seen in other tissues as well. Using high performance liquid chromatography, pentosidine contents were determined in whole vitreous bodies and in separate parts of vitreous bodies, which were all obtained from human donor eyes. Our results show that pentosidine accumulates in the human vitreous. From the rate of accumulation we could roughly estimate that vitreous collagen has as a similar or shorter half-life compared to skin collagen. This supports the concept of collagen turnover in the vitreous. In general, the female vitreous experiences a faster pentosidine accumulation than the male vitreous, and most of the pentosidine accumulation in the former occurs after 50 years of age. © 2009 Elsevier Ltd. All rights reserved. Subject BiologyBiomedical Researchadvanced glycation end productagingcollagengenderoxidative stresspentosidinevitreousadvanced glycation end productcollagenpentosidineadolescentadultagedagingarticlebioaccumulationcollagen degradationcontrolled studycorrelation analysisfemalehumanhuman tissuemaleoxidative stresspriority journalsex differencevitreous body To reference this document use: http://resolver.tudelft.nl/uuid:15de2a9b-bca0-4052-ba84-31fde6784502 DOI https://doi.org/10.1016/j.exer.2009.01.004 TNO identifier 241569 ISSN 0014-4835 Source Experimental Eye Research, 88 (6), 1043-1050 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.