Title
Pentosidine accumulates in the aging vitreous body: A gender effect
Author
van Deemter, M.
Ponsioen, T.L.
Bank, R.A.
Snabel, J.M.M.
van der Worp, R.J.
Hooymans, J.M.M.
Los, L.I.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
The human vitreous body undergoes structural changes with aging. This can be followed by a posterior vitreous detachment, which can result in ocular pathology. As in many collagenous tissues, age-related changes in the vitreous could be caused by the formation of advanced glycation end products (AGEs). The goal of this study was to find out whether the AGE pentosidine accumulates in the human vitreous with aging. With this data we were able to estimate the half-life of vitreous collagen. Furthermore, we analyzed whether there was a gender difference in pentosidine accumulation, as this was seen in other tissues as well. Using high performance liquid chromatography, pentosidine contents were determined in whole vitreous bodies and in separate parts of vitreous bodies, which were all obtained from human donor eyes. Our results show that pentosidine accumulates in the human vitreous. From the rate of accumulation we could roughly estimate that vitreous collagen has as a similar or shorter half-life compared to skin collagen. This supports the concept of collagen turnover in the vitreous. In general, the female vitreous experiences a faster pentosidine accumulation than the male vitreous, and most of the pentosidine accumulation in the former occurs after 50 years of age. © 2009 Elsevier Ltd. All rights reserved.
Subject
Biology
Biomedical Research
advanced glycation end product
aging
collagen
gender
oxidative stress
pentosidine
vitreous
advanced glycation end product
collagen
pentosidine
adolescent
adult
aged
aging
article
bioaccumulation
collagen degradation
controlled study
correlation analysis
female
human
human tissue
male
oxidative stress
priority journal
sex difference
vitreous body
To reference this document use:
http://resolver.tudelft.nl/uuid:15de2a9b-bca0-4052-ba84-31fde6784502
DOI
https://doi.org/10.1016/j.exer.2009.01.004
TNO identifier
241569
ISSN
0014-4835
Source
Experimental Eye Research, 88 (6), 1043-1050
Document type
article