Title
A combined transcriptomics and lipidomics analysis of subcutaneous, epididymal and mesenteric adipose tissue reveals marked functional differences
Author
TNO Kwaliteit van Leven
Caesar, R.
Manieri, M.
Kelder, T.
Boekschoten, M.
Evelo, C.
Müller, M.
Kooistra, T.
Cinti, S.
Kleemann, R.
Drevon, C.A.
Publication year
2010
Abstract
Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes and surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) and epididymal adipose tissue (EWAT) during high-fat feeding of male transgenic ApoE3Leiden mice with histology, targeted lipidomics and biochemical analyses of metabolic pathways to identify differentially regulated processes and site-specific functions. EWAT was found to exhibit physiological zonation. De novo lipogenesis in fat proximal to epididymis was stably low, whereas de novo lipogenesis distal to epididymis and at other locations was down-regulated in response to high-fat diet. The contents of linoleic acid and a-linolenic acid in EWAT were increased compared to other depots. Expression of the androgen receptor (Ar) was higher in EWAT than in MWAT and SWAT. We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alinolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis. © 2010 Caesar et al.
Subject
Biomedical Research
Acetyl coenzyme A
Adenosine triphosphate citrate lyase
Androgen receptor
Carbohydrate responsive element binding protein
Fatty acid synthase
Linoleic acid
Linolenic acid
Monounsaturated fatty acid
Omega 3 fatty acid
Omega 6 fatty acid
Transcription factor
Unclassified drug
Abdominal fat
Adipocyte
Animal cell
Animal tissue
Controlled study
Down regulation
Epididymis fat
Fat intake
Fatty acid desaturation
Fatty acid synthesis
Gene expression regulation
Lipid diet
Lipid metabolism
Lipid storage
Lipidomics
Lipogenesis
Male
Microarray analysis
Mouse
Nonhuman
Spermatogenesis
Subcutaneous fat
Transcriptomics
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http://resolver.tudelft.nl/uuid:15120436-f63a-4cb2-8a5e-700a278e1c9a
TNO identifier
409283
ISSN
1932-6203
Source
PLoS ONE, 5 (5), 1-14
Document type
article