Print Email Facebook Twitter Endogenous interleukin-10 protects against hepatic steatosis but does not improve insulin sensitivity during high-fat feeding in mice Title Endogenous interleukin-10 protects against hepatic steatosis but does not improve insulin sensitivity during high-fat feeding in mice Author den Boer, M.A.M. Voshol, P.J. Schröder-van Der Elst, J.P. Korsheninnikova, E. Ouwens, D.M. Kuipers, F. Havekes, L.M. Romijn, J.A. TNO Kwaliteit van Leven Publication year 2006 Abstract Several studies have demonstrated an association in humans between plasma levels or production capacity of the antiinflammatory cytokine IL-10 and insulin sensitivity. The aim of our study was to investigate the protective role of endogenous IL-10 availability in the development of diet-induced insulin resistance. We compared parameters of glucose and lipid metabolism between IL-10-/- mice and wild-type (wt) mice fed a high-fat diet for 6 wk. This diet has previously been shown to induce steatosis and insulin resistance. After 6 wk on the high-fat diet, no differences in body weight, basal metabolism (measured by indirect calorimetry), or plasma levels of glucose, triglycerides, or cholesterol were observed between IL-10-/- and wt mice. Nonetheless, in IL-10-/- mice, plasma free fatty acid levels were 75% increased compared with wt mice after overnight fasting (P < 0.05). In addition, hepatic triglyceride content was 54% increased in IL-10-/- mice (P < 0.05). During a hyperinsulinemic euglycemic clamp, no differences were observed in whole-body or hepatic insulin sensitivity between both groups. We conclude that basal IL-10 production protects against hepatic steatosis but does not improve hepatic or whole-body insulin sensitivity, during high-fat feeding. Copyright © 2006 by The Endocrine Society. Chemicals / CAS: amyloid A protein, 59165-71-8; fibrinogen, 9001-32-5; protein kinase B, 148640-14-6; Blood Glucose; Dietary Fats; Fibrinogen, 9001-32-5; Glucose, 50-99-7; Interleukin-10, 130068-27-8; Lipids; Serum Amyloid A Protein Subject BiologyBiomedical ResearchAmyloid A proteinfibrinogeninterleukin 10protein kinase BAnimal experimentCalorimetryCholesterol blood levelControlled studyExperimental modelFatty liverFeedingGlucose blood levelHlucose metabolismHigh performance liquid chromatographyHyperinsulinemiaInsulin sensitivityLipid dietLipid metabolismMouseNonhumanPhosphorylationSteatosisTriacylglycerol blood levelAdipose TissueAnimalsBlood GlucoseChromatography, Thin LayerDietDietary FatsEnergy MetabolismFibrinogenGenotypeGlucoseGlucose Clamp TechniqueInsulin ResistanceInterleukin-10Lipid MetabolismLipidsLiver DiseasesMaleMiceMice, Inbred C57BLMice, KnockoutPhenotypeSerum Amyloid A Protein To reference this document use: http://resolver.tudelft.nl/uuid:1466fe14-82a5-4736-b91e-e89ce3d2f525 DOI https://doi.org/10.1210/en.2006-0417 TNO identifier 239484 ISSN 0013-7227 Source Endocrinology, 147 (10), 4553-4558 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.