Title
Endogenous interleukin-10 protects against hepatic steatosis but does not improve insulin sensitivity during high-fat feeding in mice
Author
den Boer, M.A.M.
Voshol, P.J.
Schröder-van Der Elst, J.P.
Korsheninnikova, E.
Ouwens, D.M.
Kuipers, F.
Havekes, L.M.
Romijn, J.A.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
Several studies have demonstrated an association in humans between plasma levels or production capacity of the antiinflammatory cytokine IL-10 and insulin sensitivity. The aim of our study was to investigate the protective role of endogenous IL-10 availability in the development of diet-induced insulin resistance. We compared parameters of glucose and lipid metabolism between IL-10-/- mice and wild-type (wt) mice fed a high-fat diet for 6 wk. This diet has previously been shown to induce steatosis and insulin resistance. After 6 wk on the high-fat diet, no differences in body weight, basal metabolism (measured by indirect calorimetry), or plasma levels of glucose, triglycerides, or cholesterol were observed between IL-10-/- and wt mice. Nonetheless, in IL-10-/- mice, plasma free fatty acid levels were 75% increased compared with wt mice after overnight fasting (P < 0.05). In addition, hepatic triglyceride content was 54% increased in IL-10-/- mice (P < 0.05). During a hyperinsulinemic euglycemic clamp, no differences were observed in whole-body or hepatic insulin sensitivity between both groups. We conclude that basal IL-10 production protects against hepatic steatosis but does not improve hepatic or whole-body insulin sensitivity, during high-fat feeding. Copyright © 2006 by The Endocrine Society. Chemicals / CAS: amyloid A protein, 59165-71-8; fibrinogen, 9001-32-5; protein kinase B, 148640-14-6; Blood Glucose; Dietary Fats; Fibrinogen, 9001-32-5; Glucose, 50-99-7; Interleukin-10, 130068-27-8; Lipids; Serum Amyloid A Protein
Subject
Biology
Biomedical Research
Amyloid A protein
fibrinogen
interleukin 10
protein kinase B
Animal experiment
Calorimetry
Cholesterol blood level
Controlled study
Experimental model
Fatty liver
Feeding
Glucose blood level
Hlucose metabolism
High performance liquid chromatography
Hyperinsulinemia
Insulin sensitivity
Lipid diet
Lipid metabolism
Mouse
Nonhuman
Phosphorylation
Steatosis
Triacylglycerol blood level
Adipose Tissue
Animals
Blood Glucose
Chromatography, Thin Layer
Diet
Dietary Fats
Energy Metabolism
Fibrinogen
Genotype
Glucose
Glucose Clamp Technique
Insulin Resistance
Interleukin-10
Lipid Metabolism
Lipids
Liver Diseases
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Phenotype
Serum Amyloid A Protein
To reference this document use:
http://resolver.tudelft.nl/uuid:1466fe14-82a5-4736-b91e-e89ce3d2f525
DOI
https://doi.org/10.1210/en.2006-0417
TNO identifier
239484
ISSN
0013-7227
Source
Endocrinology, 147 (10), 4553-4558
Document type
article