Print Email Facebook Twitter Effects of alkali treated proteins: Feeding studies with free and protein bound lysinoalanine in rats and other animals Title Effects of alkali treated proteins: Feeding studies with free and protein bound lysinoalanine in rats and other animals Author de Groot, A.P. Slump, P. Feron, V.J. van Beek, L. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1976 Abstract To find out whether alkali treated proteins possess nephrotoxic properties, feeding studies were conducted with drastically treated soybean protein and casein, and also with lysinoalanine (LAL), the amino acid known to be formed in proteins subjected to high pH at an elevated temperature. The feeding of synthesized LAL to rats at dietary levels of 100 ppm and above induced typical renal changes, called nephrocytomegalia. No such changes or any other indications of toxicity were observed, however, upon feeding much higher levels of LAL (up to 6,000 ppm) when provided as the protein bound compound in alkali treated casein or soybean protein. When set free by complete acid hydrolysis, LAL induced considerable renal activity, comparable to that of the synthetic compound. These results indicate that alkali treatment of proteins does not induce nephrotoxic properties provided that the compound remains protein bound. Some nephrotoxic activity was observed, however, with peptide bound LAL in break down products (molecular weight < 5,000) of alkali treated casein, but considerably less than that of the free compound. LAL analyses in blood, urine, and feces of rats fed free or protein bound LAL indicated a positive correlation between intestinal absorption and nephrotoxic potential. No renal changes were encountered upon feeding diets with 1,000 ppm synthetic LAL to mice, hamsters, rabbits, quail, dogs or monkeys, which suggest a species specificity of LAL induced renal changes in rats. Chemicals/CAS: lysinoalanine, 18810-04-3, 23250-50-2; protein, 67254-75-5; Caseins; Dipeptides; Vegetable Proteins Subject LysinoalanineProteinFeeding behaviorTheoretical studyToxicityAnimalCaseinsCoturnixDipeptidesDogsFemaleHamstersHaplorhiniHydrogen-Ion ConcentrationKidneyMacaca mulattaMaleMesocricetusMiceRabbitsRatsSoybeansSpecies SpecificityVegetable Proteins To reference this document use: http://resolver.tudelft.nl/uuid:13c47c12-6a0b-493e-ae47-20ee72dc6e8c TNO identifier 228029 ISSN 0022-3166 Source Journal of Nutrition, 106 (10), 1527-1538 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.