Print Email Facebook Twitter Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry Title Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry Author Rose, R.J. Thompson, N.J. Van Duijn, E. Damoc, E. Denisov, E. Makarov, A. Heck, A.J.R. Publication year 2012 Abstract Monoclonal antibodies (mAbs) represent a major class of therapeutic biomolecules being developed by the biopharmaceutical industry, their popularity largely being a result of their high specificity towards targets. Therapeutic mAbs are being developed for various diseases, with a strong focus on cancer and immunological disorders. All antibody drugs currently approved for clinical use are based on the immunoglobulin G (IgG) class; these molecules are composed of two heavy chains and two light chains, connected by disulfide bonds, and with a molecular weight of approximately 150 kDa. Antibody-based biopharmaceuticals can be complex and heterogeneous molecules, and a range of analytical tools are necessary to characterize them fully. Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesBiomedical InnovationPharmacology NutritionHealthy LivingAnalytical methodAnalytical toolAntibody drugsBiopharmaceutical industryBiopharmaceuticalsClinical useDisulfide bondsHeavy chainHigh specificityImmunoglobulin GLight chainMonoclonal antibodies (mAbs)Orbitrap To reference this document use: http://resolver.tudelft.nl/uuid:1095d3ff-c043-47d7-b4c1-65d411ac1fde DOI https://doi.org/10.1002/anie.201206745 TNO identifier 526121 Source Angewandte Chemie - International Edition, 51 (52), 12992-12996 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.