Title
Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry
Author
Rose, R.J.
Thompson, N.J.
Van Duijn, E.
Damoc, E.
Denisov, E.
Makarov, A.
Heck, A.J.R.
Publication year
2012
Abstract
Monoclonal antibodies (mAbs) represent a major class of therapeutic biomolecules being developed by the biopharmaceutical industry, their popularity largely being a result of their high specificity towards targets. Therapeutic mAbs are being developed for various diseases, with a strong focus on cancer and immunological disorders.[1] All antibody drugs currently approved for clinical use are based on the immunoglobulin G (IgG) class; these molecules are composed of two heavy chains and two light chains, connected by disulfide bonds, and with a molecular weight of approximately 150 kDa. Antibody-based biopharmaceuticals can be complex and heterogeneous molecules, and a range of analytical tools are necessary to characterize them fully.
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Pharmacology Nutrition
Healthy Living
Analytical method
Analytical tool
Antibody drugs
Biopharmaceutical industry
Biopharmaceuticals
Clinical use
Disulfide bonds
Heavy chain
High specificity
Immunoglobulin G
Light chain
Monoclonal antibodies (mAbs)
Orbitrap
To reference this document use:
http://resolver.tudelft.nl/uuid:1095d3ff-c043-47d7-b4c1-65d411ac1fde
DOI
https://doi.org/10.1002/anie.201206745
TNO identifier
526121
Source
Angewandte Chemie - International Edition, 51 (52), 12992-12996
Document type
article