Title
A study of the variation in the salivary peptide profiles of young healthy adults acquired using MALDI-TOF MS
Author
Prodan, A.
Brand, H.
Imangaliyev, S.
Tsivtsivadze, E.
van der Weijden, F.
de Jong, A.
Paauw, A.
Crielaard, W.
Keijser, B.
Veerman, E.
Publication year
2016
Abstract
A cross-sectional observational study was conducted to evaluate the inter-individual variation in the MALDI-TOF MS peptide profiles of unstimulated whole saliva in a population of 268 systemically healthy adults aged 18-30 yr (150 males and 118 females) with no apparent caries lesions or periodontal disease. Using Spectral Clustering, four subgroups of individuals were identified within the study population. These subgroups were delimited by the pattern of variation in 9 peaks detected in the 2-15 kDa m/z range. An Unsupervised Feature Selection algorithm showed that P-C peptide, a 44 residue-long salivary acidic proline-rich protein, and three of its fragments (Fr. 1-25, Fr. 15-35 and Fr. 15-44) play a central role in delimiting the subgroups. Significant differences were found in the salivary biochemistry of the subgroups with regard to lysozyme and chitinase, two enzymes that are part of the salivary innate defense system (p < 0.001). These results suggest that MALDI-TOF MS salivary peptide profiles may relate information on the underlying state of the oral ecosystem and may provide a useful reference for salivary disease biomarker discovery studies. © 2016 Prodan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Subject
Observation, Weapon & Protection Systems
CBRN - CBRN Protection
TS - Technical Sciences
Biology
To reference this document use:
http://resolver.tudelft.nl/uuid:0eeff430-b3f9-431a-9773-498a141909fd
DOI
https://doi.org/10.1371/journal.pone.0156707
TNO identifier
537423
Publisher
Public Library of Science
ISSN
1932-6203
Source
PLoS ONE, 11 (6)
Article number
e0156707
Document type
article