Title
In vitro fermentability of sugar beet pulp derived oligosaccharides using human and pig fecal inocula
Author
Leijdekkers, A.G.M.
Aguirre, M.
Venema, K.
Bosch, G.
Gruppen, H.
Schols, H.A.
Publication year
2014
Abstract
The in vitro fermentation characteristics of different classes of sugar beet pectic oligosaccharides (SBPOS) were studied using human and pig fecal inocula. The SBPOS consisted mainly of partially acetylated rhamnogalacturonan oligosaccharides and partially methyl-esterified/acetylated homogalacturonan oligosaccharides. Some SBPOS contained an unsaturated galacturonic acid residue at their non-reducing end. It was shown that SBPOS could be completely fermented by human and pig fecal microbiota, thereby producing butyrate yet mainly acetate and propionate as metabolites. The degradation of SBPOS by pig fecal microbiota was different and much slower compared to human fecal microbiota. In general, rhamnogalacturonan oligosaccharides were degraded slower than homogalacturonan oligosaccharides. Acetylation of rhamnogalacturonan oligosaccharides lowered the degradation rate by pig fecal microbiota but not by human fecal microbiota. No classic bifidogenic effect was shown for SBPOS using human fecal inoculum. However, several other potentially interesting modifications in the microbiota composition that can be associated with host health were observed, which are discussed. © 2014 American Chemical Society.
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Fecal microbiota
Mass spectrometry
Pectic oligosaccharides
Prebiotics
Short-chain fatty acids
To reference this document use:
http://resolver.tudelft.nl/uuid:0e991cec-1b4c-4d20-8568-2de212966c47
DOI
https://doi.org/10.1021/jf4049676
TNO identifier
489150
ISSN
0021-8561
Source
Journal of Agricultural and Food Chemistry, 62 (5), 1079-1087
Document type
article