Title
Effect of diesel exhaust generated by a city bus engine on stress responses and innate immunity in primary bronchial epithelial cell cultures
Author
Zarcone, M.C.
Duistermaat, E.
Alblas, M.J.
van Schadewijk, A.
Ninaber, D.K.
Clarijs, V.
Moerman, M.M.
Vaessen, D.
Hiemstra, P.S.
Kooter, I.M.
Publication year
2018
Abstract
Harmful effects of diesel emissions can be investigated via exposures of human epithelial cells, but most of previous studies have largely focused on the use of diesel particles or emission sources that are poorly representative of engines used in current traffic. We studied the cellular response of primary bronchial epithelial cells (PBECs) at the air-liquid interface (ALI) to the exposure to whole diesel exhaust (DE) generated by a Euro V bus engine, followed by treatment with UVinactivated non-typeable Haemophilus influenzae (NTHi) bacteria to mimic microbial exposure. The effect of prolonged exposures was investigated, as well as the difference in the responses of cells from COPD and control donors and the effect of emissions generated during a cold start. HMOX1 and NQO1 expression was transiently induced after DE exposure. DE inhibited the NTHi-induced expression of human beta-defensin-2 (DEFB4A) and of the chaperone HSPA5/BiP. In contrast, expression of the stress-induced PPP1R15A/GADD34 and the chemokine CXCL8 was increased in cells exposed to DE and NTHi. HMOX1 induction was significant in both COPD and controls, while inhibition of DEFB4A expression by DE was significant only in COPD cells. No significant differences were observed when comparing cellular responses to cold engine start and prewarmed engine emissions.
Subject
Health
Healthy for Life
Healthy Living
2015 Observation, Weapon & Protection Systems Urban Mobility & Environment
CBRN - CBRN Protection EMS - Environmental Modelling, Sensing & Analysis
ELSS - Earth, Life and Social Sciences
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http://resolver.tudelft.nl/uuid:0cb43b24-bedd-47a7-8644-791024b18de6
TNO identifier
787166
Source
Toxicology in Vitro, 48 (48), 221-231
Document type
article